| 小麦F-box蛋白基因的电子克隆和生物信息学分析 |
| |
| 引用本文： | 王俊生,李俐俐,杨欢,谭光轩,殷贵鸿.小麦F-box蛋白基因的电子克隆和生物信息学分析[J].河南农业科学,2013,42(2). |
| |
| 作者姓名： | 王俊生 李俐俐 杨欢 谭光轩 殷贵鸿 |
| |
| 作者单位： | 周口师范学院生命科学系,河南周口,466001 |
| |
| 基金项目： | 周口师范学院大学生创新基金 |
| |
| 摘 要： | 为探讨F-box蛋白在小麦发育过程中的调节作用,利用电子克隆技术获得了一个小麦F-box蛋白基因,采用生物信息学方法,对其编码的蛋白质从序列特征、理化性质、跨膜结构域、亚细胞定位、高级结构及功能域等方面进行了预测和分析。结果表明,小麦F-box蛋白由362个氨基酸组成,在N端和C端分别存在一个F-box保守结构域和一个DUF295保守结构域(功能未知),该蛋白质二级结构中以α-螺旋(23.48%)、扩展束(25.41%)和无规则卷曲(47.24%)为主,在94-115位点处可能存在一个由内向外的跨膜区,N端不存在信号肽序列,亚细胞定位于细胞质中;该F-box蛋白与二穗短柄草、玉米和水稻的F-box蛋白相似性较高,进化距离较近。
|
| 关 键 词： | 小麦 F-box蛋白基因 电子克隆 生物信息学 |
Electronic Clone and Bioinformatics Analysis of F-box Protein Gene from Wheat(Triticum aestivum L.) |
| |
| WANG Jun-sheng
,
LI Li-li
,
YANG Huan
,
TAN Guang-xuan
,
YIN Gui-hong.Electronic Clone and Bioinformatics Analysis of F-box Protein Gene from Wheat(Triticum aestivum L.)[J].Journal of Henan Agricultural Sciences,2013,42(2). |
| |
| Authors: | WANG Jun-sheng LI Li-li YANG Huan TAN Guang-xuan YIN Gui-hong |
| |
| Abstract: | In the paper,an F-box protein gene from wheat was obtained by electronic clone technique,and some characters of the F-box protein were analyzed and predicted by bioinformatics method,including the composition of amino acid sequence,hydrophobicity,secondary structure and function of protein etc.The results showed that the F-box protein was composed of 362 amino acids,and contained an F-box domain in N end and a DUF295 conserved domain in C end of protein sequence.The secondary structure of the F-box protein mainly included α-helix(23.48%),extended strand(25.41%) and random coil(47.24%).The F-box protein had higher sequece indentities and closer evolution distance with that from other Gramineae plants including Brachypodium distachyon,Oryza sativa and Zea may. |
| |
| Keywords: | Triticum aestivum L F-box protein gene electronic clone bioinformatics |
| 本文献已被 万方数据 等数据库收录！ |
|
