| 动物组织中β-兴奋剂类药物多残留酶联免疫检测方法的建立 |
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| 引用本文： | 万宇平,刘宁,陶光灿,吴鹏,李勇,崔廷婷.动物组织中β-兴奋剂类药物多残留酶联免疫检测方法的建立[J].河南农业科学,2013,42(2). |
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| 作者姓名： | 万宇平 刘宁 陶光灿 吴鹏 李勇 崔廷婷 |
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| 作者单位： | 1. 北京勤邦生物技术有限公司,北京,102206
2. 临沂新程金锣肉制品集团有限公司,山东临沂,276036 |
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| 摘 要： | 应用直接竞争酶联免疫吸附技术,建立了一种快速检测动物组织中β-兴奋剂类药物多残留的方法,并对其技术性能进行了评价。结果表明,该方法的IC50浮动范围为0.4～0.7μg/L,对组织样本的检测限为0.5μg/kg;样本添加回收率为72.9%～103.5%,变异系数为5.8%～14.1%;可用于检测克仑特罗、沙丁胺醇、塞布特罗、妥布特罗、克仑潘特、溴布特罗、溴氯布特罗、马喷特罗。
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| 关 键 词： | 酶联免疫吸附法 β-兴奋剂类药物 多残留 动物组织 |
Determination of β-agonists Multi-residue in Animal Tissue by Enzyme-linked Immunosorbent Assay |
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| WAN Yu-ping
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LIU Ning
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TAO Guang-can
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WU Peng
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LI Yong
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CUI Ting-ting.Determination of β-agonists Multi-residue in Animal Tissue by Enzyme-linked Immunosorbent Assay[J].Journal of Henan Agricultural Sciences,2013,42(2). |
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| Authors: | WAN Yu-ping LIU Ning TAO Guang-can WU Peng LI Yong CUI Ting-ting |
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| Abstract: | A rapid method for detection of β-agonists multi-residue in animal tissue was established based on direct competitive enzyme-linked immunosorbent assay(ELISA).The results showed that the IC50 was 0.4-0.7 μg/L,and the detection limit for animal tissue was 0.5 μg/kg.Sample recoveries were 72.9%-103.5% and the coefficients of variation were 5.8%-14.1%.This method was suitable for detecting clenbuterol,salbutamol,cimbuterol,tulobuterol,clenpenterol,brombuterol,bromchlorbuterol,mapenterol.This method is rapid,sensitive,and reliable,which provides a convenient and accurate method for detection of β-agonists residue. |
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| Keywords: | ELISA β-agonists multi-residue animal tissue |
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