| 猪链球菌2型基因芯片检测技术的建立 |
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| 引用本文： | 刘玲玲,李文刚,唐桂芬,程亚楼.猪链球菌2型基因芯片检测技术的建立[J].河南农业科学,2013,42(2). |
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| 作者姓名： | 刘玲玲 李文刚 唐桂芬 程亚楼 |
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| 作者单位： | 1. 郑州牧业工程高等专科学校,河南郑州,450011
2. 河南农业大学,河南郑州,450002 |
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| 摘 要： | 选取猪链球菌2型的cps2J基因设计引物和探针,通过PCR扩增Cy3标记的DNA片段与固定于芯片上的探针进行杂交,并将杂交完毕的芯片进行信号扫描分析,根据荧光信号的强度来确定是否存在猪链球菌2型。结果表明,采用浓度为5μmol/L的探针与PCR产物杂交30min即可得到清晰的荧光信号,表明基因芯片检测技术是一种灵敏度高、特异好的检测方法。该方法的建立可以快速有效地对猪链球菌2型做出诊断,具有较好的应用前景。
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| 关 键 词： | 猪链球菌2型 基因芯片 杂交 检测方法 |
Establishment of Gene Chip Method for Detecting Streptococcus suis Serotype 2 |
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| LIU Ling-ling
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LI Wen-gang
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TANG Gui-fen
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CHENG Ya-lou.Establishment of Gene Chip Method for Detecting Streptococcus suis Serotype 2[J].Journal of Henan Agricultural Sciences,2013,42(2). |
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| Authors: | LIU Ling-ling LI Wen-gang TANG Gui-fen CHENG Ya-lou |
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| Abstract: | The cps2J gene of Streptococcus suis serotype 2 was chosen as a target for the design of primers and probes.Amplified DNA fragments labeled by Cy3 were hybridized with the gene chip fixed with specific probes.Then whether the Streptococcus suis serotype 2 was in or not was detected by scanning the signal obtained from the gene chip and the intensity of fluorescent signal.The method that adopt 5 μmol/L of probe to hybridize with PCR product hybrid for 30 min can get clear fluorescent signal.This indicates that this method is a sensitive and specific method for detecting the disease of Streptococcus suis serotype 2. |
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| Keywords: | Streptococcus suis serotype 2 gene chip hybrid detection method |
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