Detection of genes encoding for virulence and adherence factors in Escherichia coli isolated in slaughtered Sarda breed sheep |
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| Authors: | Busia Gianluca Mureddu Anna Mazza Roberta Meloni Domenico Consolati Simonetta G Piras Francesca Burgess Catherine M Duffy Geraldine R Mazzette |
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| Institution: | 1. Veterinary Medicine Department, University of Sassari, via Vienna 2, 07100 Sassari, Italy;2. Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland |
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| Abstract: | In order to investigate the pathogenic profile of Escherichia coli hosted in “Sarda” sheep, autochthonous race present in Sardinia, thirty-seven E. coli strains collected from different sources (fleeces, carcass swabs and gut mucosa) of pre-chill slaughtered sheep (ewes and lambs) were serotyped using pheno- and genotypic methods. Furthermore, the presence of genes encoding for virulence factors and mediating for localized mucosal adherence factors was investigated, and pulsed-field gel electrophoresis (PFGE) characterization was performed. |
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| Keywords: | Escherichia coli Sheep Serotyping Associated virulence factors PCR |
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