| 甜杏仁中苦杏仁苷质量分数的HPLC定量分析 |
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| 引用本文： | 张玲,翟明普,景淼.甜杏仁中苦杏仁苷质量分数的HPLC定量分析[J].果树学报,2009,26(2). |
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| 作者姓名： | 张玲 翟明普 景淼 |
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| 作者单位： | 张玲,ZHANG Ling(省部共建森林培育与保护教育部重点实验室,北京林业大学,北京,100083;北京市第卜四巾学,北京,100053);翟明普,景淼,ZHAI Ming-pu,JING Miao(省部共建森林培育与保护教育部重点实验室,北京林业大学,北京,100083)
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| 摘 要： | 杏仁中所含的苦杏仁苷具有一定的生物毒性,从林产品安全的角度,采用HPLC定量分析方法对3个仁用杏品种杏仁中的苦杏仁苷质量分数进行了检测,旨在为仁用杏杏仁的食用安全性评价提供科学依据。结果表明,以纯甲醇作为提取溶剂,按照1∶20的料(脱脂粉末)液比,80℃水浴回流提取1.5h,为仁用杏杏仁中苦杏仁苷提取的最优化方法;采用HPLC法测得3个仁用杏品种木瓜杏、龙王帽、薄壳1号杏仁中苦杏仁苷质量分数分别为0.138%,0.154%和0.123%;测得的对照(中药材苦杏仁)中苦杏仁苷质量分数为2.269%。
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| 关 键 词： | 仁用杏 苦杏仁苷 HPLC 定量分析 |
Quantitative analysis of amygdalin in the kernel of kernel-using apricot cultivars by HPLC method |
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| ZHANG Ling,ZHAI Ming-pu,JING Miao.Quantitative analysis of amygdalin in the kernel of kernel-using apricot cultivars by HPLC method[J].Journal of Fruit Science,2009,26(2). |
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| Authors: | ZHANG Ling ZHAI Ming-pu JING Miao |
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| Institution: | 1Key laboratory for Silivicuhure and Conservation of Ministry of Education;Beijing Forestry University;Beijing 100083 China;2Beijing No.14 High School;Beijing 100053 China |
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| Abstract: | Amygdalin in kernel-using apricot cultivars has bio-toxicity to some extent.For the security of forest products and standard indices of security to eat of the 3 kernel-using apricot cultivars,the contents of amygdalin in the kernels were tested by the method of HPLC(High Performance Liquid Chromatographic).It was proved that the optimal condition for extracting amygdalin from apricot kernels was using methanol as flux,fine powder(apricot kernel without fat) and liquid in a propor-tion of 1 to 20 and extract... |
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| Keywords: | HPLC |
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