| 观赏羽衣甘蓝高频再生体系的建立 |
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| 引用本文： | 赵秀枢,李名扬,张文玲,刘凡.观赏羽衣甘蓝高频再生体系的建立[J].广西农业生物科学,2009(1). |
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| 作者姓名： | 赵秀枢 李名扬 张文玲 刘凡 |
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| 作者单位： | 西南大学园艺园林学院;国家蔬菜工程技术研究中心; |
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| 基金项目： | 国家863项目(2008AA10Z155);;北京市科技合同项目(Z07070501770704)资助 |
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| 摘 要： | 观赏羽衣甘蓝是一种赏食兼用的耐冻优良景观植物。为了通过基因工程手段赋予其抗虫性,并进一步提高其低温耐受性,获得新的抗性种质,本研究以优良育种品系为试材,详细探讨了影响离体培养不定芽再生的因素,建立了高效再生体系。以8份不同类型羽衣甘蓝基因型的带柄子叶、子叶、下胚轴为外植体,研究了不同基因型、不同外植体、不同的培养基激素浓度配比及添加AgNO3对不定芽诱导的影响。结果表明:无论基因型及使用的培养基,带柄子叶均为最佳外植体;不同的基因型、不同外植体其最佳的芽诱导培养基不同;筛选出两份材料,其带柄子叶分别在L6(MS+6-BA1.0mg/L+NAA0.1mg/L)及L3(MS+6-BA1.5mg/L)培养基中不定芽诱导率为100%,下胚轴诱导率最高也能达88.33%;培养基中添加4mg/L AgNO3不利于羽衣甘蓝的不定芽诱导;再生株生根以MS+NAA0.1mg/L效果好,生根率可达100%。
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| 关 键 词： | 羽衣甘蓝 离体培养 外植体 不定芽 |
Establishment of High Adventitious Shoot Regeneration System of Ornamental Kale |
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| Zhao Xiushu , Li Mingyang Zhang Wenling , Liu Fan College of Horticulture , L,scape,Southwest University,Chongqing, National Engineering Research Center for Vegetable,Beijing.Establishment of High Adventitious Shoot Regeneration System of Ornamental Kale[J].Journal of Guangxi Agricultural and Biological Science,2009(1). |
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| Authors: | Zhao Xiushu Li Mingyang Zhang Wenling Liu Fan College of Horticulture L scape Southwest University Chongqing National Engineering Research Center for Vegetable Beijing |
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| Institution: | Zhao Xiushu 1,2 Li Mingyang 1 Zhang Wenling 1,2 Liu Fan 2 1 College of Horticulture , L,scape,Southwest University,Chongqing,400715,2 National Engineering Research Center for Vegetable,Beijing,100097 |
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| Abstract: | Ornamental kale(Brassica oleracea var.acephala) is an edible freezing tolerant excellent landscape plant.To endow it with pest insect resistance and increase the minus temperature tolerance further,genetic engineering will be operated.In this research,the elite breeding lines of ornamental kale were selected as the material,and factors affecting the adventitious shoot regeneration in vitro were studied in detail.A high efficient shoot regeneration system was established.Different explants of eight genotypes... |
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| Keywords: | Brassica oleracea var acephala In vitro culture Explant Adventitious shoot |
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