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| Cu2+对缢蛏稚贝的急性毒性及对抗氧化酶活力和丙二醛含量的影响 | |
| 引用本文： | 刘浩明,董迎辉,霍礼辉,林志华,王昭萍.Cu2+对缢蛏稚贝的急性毒性及对抗氧化酶活力和丙二醛含量的影响[J].中国水产科学,2012,19(1):182-187. |
| 作者姓名： | 刘浩明 董迎辉 霍礼辉 林志华 王昭萍 |
| 作者单位： | 1. 中国海洋大学水产学院,山东青岛266003;浙江万里学院生物与环境学院,浙江宁波315100 2. 浙江万里学院生物与环境学院,浙江宁波,315100 3. 中国海洋大学水产学院,山东青岛,266003 |
| 基金项目： | 国家科技支撑计划项目，国家现代农业产业技术体系建设项目 |
| 摘 要： | 研究了Cu2+对缢蛏稚贝的急性毒性并进行安全评价;根据96 h LC50值设定10、30、40μg/L 3个Cu2+质量浓度梯度进行暴露实验,以缢蛏(Sinonovacula constricta Lamark)稚贝软体组织中过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性和脂质过氧化物丙二醛(MDA)含量为指标,研究Cu2+污染对缢蛏稚贝的毒性作用。结果表明,Cu2+对缢蛏稚贝96 hLC50为309.742μg/L;不同浓度Cu2+胁迫下,96 h内缢蛏稚贝抗氧化酶(CAT、SOD)活力发生显著的变化(P<0.05),除最高组40μg/L在48 h时CAT活力被显著抑制(P<0.05)外,96 h内绝大部分时间点与沙滤海水对照组相比,各暴露组抗氧化酶(CAT、SOD)活性处于诱导状态;96 h内各暴露组缢蛏稚贝丙二醛(MDA)含量均明显高于对照组(P<0.05)。研究认为,CAT、SOD活性及MDA含量变化可反映缢蛏稚贝受Cu2+伤害的程度,可用作缢蛏安全性风险评价的参考依据。 |
| 关 键 词： | Cu2+ 缢蛏 稚贝 CAT SOD MDA |
| 修稿时间： | 2012/2/2 0:00:00 |
Acute toxicity of Cu2+ and its effects on antioxidant enzymes in Sinonovacula constricta juveniles |
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| LIU Haoming,DONG Yinghui,HUO Lihui,LIN Zhihu,WANG Zhaoping.Acute toxicity of Cu2+ and its effects on antioxidant enzymes in Sinonovacula constricta juveniles[J].Journal of Fishery Sciences of China,2012,19(1):182-187. | |
| Authors: | LIU Haoming DONG Yinghui HUO Lihui LIN Zhihu WANG Zhaoping |
| Institution: | 1.Fishery College,Ocean University of China,Qingdao 266003,China; 2.Zhejiang Wanli University,College of Biological and Environmental Sciences,Ningbo 315100,China |
| Abstract: | We evaluated the acute toxicity of Cu2+ and its effects on antioxidant enzymes in Sinonovacula con-stricta juveniles.The results of acute toxicity test showed that the median 96 h lethal concentration(LC50) for Cu2+ was 309.74 μg/L.We also measured catalase(CAT) and superoxide dismutase(SOD) activity and malondialde-hyde(MDA) content in the soft tissue of clams exposed to 10,30,or 40 μg/L Cu2+,respectively,at 6,12,24,48,72 and 96 h of exposure.CAT activity of the maximum exposed group was lower after 48 h relative to the controls whereas both CAT and SOD activity of all exposure groups were higher at 6,24,72 and 96 h.The MDA content was higher in fish exposed to Cu2+ than in the control group after 96 h.Our results suggest that exposure to Cu2+ causes oxidative damage in S.constricta juveniles. |
| Keywords: | Cu2+ Sinonovacula constricta juveniles CAT SOD MDA |
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