| 哲罗鲑卵子和卵腔液的生化组成与其发眼率的相关性 |
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| 引用本文： | 李雪,张颖,尹家胜.哲罗鲑卵子和卵腔液的生化组成与其发眼率的相关性[J].中国水产科学,2012,19(2):223-228. |
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| 作者姓名： | 李雪 张颖 尹家胜 |
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| 作者单位： | 1. 中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070;上海海洋大学水产与生命学院,上海201306
2. 中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨,150070 |
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| 基金项目： | 农业部公益性行业(农业)科研专项(201003055);国家十二五支撑计划(2012BAD25B10) |
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| 摘 要： | 为了解哲罗鲑(Hucho taimen)卵子及卵腔液的生化组成及其与发眼率的关系,采用生物化学的方法对雌性哲罗鲑Ⅴ期卵子及卵腔液的酶、蛋白质﹑氨基酸﹑维生素和矿物质等组成和含量进行了测定,并且将各组份与哲罗鲑受精卵的发眼率进行了回归分析。结果表明:哲罗鲑Ⅴ期卵子中含有碱性磷酸酶、酸性磷酸酶、谷草转氨酶、琥珀酸脱氢酶﹑Mg2+-ATP酶﹑磷﹑钙离子﹑铁离子﹑维生素C和维生素E。卵子中Mg2+-ATP酶活力≥8.3×10-5μmol(Pi)/(min.mg prot)、磷含量在12.97~23.0 3μmol/g(卵)之间及氨基酸含量在574.89~1195.40μmol/g(卵)之间,哲罗鲑卵的发眼率均≥80%。哲罗鲑的卵腔液中含有碱性磷酸酶、酸性磷酸酶、谷草转氨酶、琥珀酸脱氢酶、磷、钙离子、铁离子、维生素C和维生素E,当谷草转氨酶酶活力≥18.14μmol/(min·L),哲罗鲑卵的发眼率≥80%。哲罗鲑Ⅴ期卵子中Mg2+-ATP酶活力、磷含量﹑氨基酸含量以及卵腔液中谷草转氨酶酶活力与其发眼率存在着显著相关性。
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| 关 键 词： | 哲罗鲑 卵子 卵腔液 生化组成 发眼率 |
| 修稿时间： | 2012/3/19 0:00:00 |
Relationship between eyed rate and the biochemical composition of eggs and ovarian fluid in Hucho taimen |
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| LI Xue,ZHANG Ying,YIN Jiasheng.Relationship between eyed rate and the biochemical composition of eggs and ovarian fluid in Hucho taimen[J].Journal of Fishery Sciences of China,2012,19(2):223-228. |
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| Authors: | LI Xue ZHANG Ying YIN Jiasheng |
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| Institution: | 1.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China; 2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China |
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| Abstract: | We investigated the relationship between the eyed rate and the biochemical composition of eggs and ovarian fluid in Hucho taimen.We measured enzyme activity and levels of protein,amino acids,vitamins,and minerals in stage V eggs and ovarian fluid.We used each of these parameters as an independent variable in a re-gression model to predict the eyed rate.There was a significant correlation between the eyed rate and Mg2+-AT-Pase activity,phosphorus levels,and amino acids in the eggs.There was no correlation between the eyed rate and the remaining parameters(protein,acid phosphatase,alkaline phosphatase,aspartate aminotransferase,succinate dehydrogenase,calcium ions,iron ions,vitamin C,and vitamin E).Egg batches with high viability(≥80%) were characterized by the following: Mg2+-ATPase activity above 8.3×10-5 μmolPi/(min·mgprot),levels of phosphorus between 12.97 and 23.03 μmol/g egg,and levels of amino acids between 574.89 and 1 195.40 μmol/g egg.In con-trast,in the ovarian fluid only aspartate aminotransferase activity was correlated with the eyed rate of H.taimen.Egg batches with high viability(≥80%) had aspartate aminotransferase activity >18.14 μmol/min/L ovarian fluid. |
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| Keywords: | Hucho taimen egg ovarian fluid biochemical composition eyed rate |
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