| 中、西蜂纯种及其不同品系蜂的基因组DNA多态性分析 |
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| 引用本文： | 王尉平,张雅娟,等.中、西蜂纯种及其不同品系蜂的基因组DNA多态性分析[J].中国养蜂,2001,52(4):12-13. |
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| 作者姓名： | 王尉平 张雅娟 |
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| 作者单位： | 王尉平（苏州大学生命科学院生化教研究,苏州,215007）
张雅娟（苏州大学生命科学院生化教研究,苏州,215007）
蒋滢（苏州大学生命科学院生化教研究,苏州,215007）
张大隆（苏州大学生命科学院生化教研究,苏州,215007）
葛凤晨（苏州大学生命科学院生化教研究,苏州,215007） |
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| 摘 要： | 使用P8(5‘-CCCACCCTTG-3‘)和W2（5‘-CGGCCCCGGT-3‘)随机引物，通过PRAPD（Random Amplified Polymorphic DNA)-PCR(polymerase chain reaction)遗传学分析，分别对中、西纯种蜂的不同品系的基因组DNA进行多态性分析。结果：中、西纯种蜂的不同品系的DNA多态4性图谱均具有明显的差异，从而为中、西纯种蜂的品系之间的鉴别提供了科学依据。
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| 关 键 词： | 西蜂 蜜蜂品系 基因组DNA多态性 聚合酶链反应 中蜂 |
| 文章编号： | 0412-4367(2001)04-0012-02 |
| 修稿时间： | 2000年11月29 |
ANALYSIS OF THE GENOMIC DNA POLYMORPHISM OF DIFFERENT RACES IN PURE KIND OF CHINESE BEES AND WESTERN BEES |
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| Wang weiping Zhang yajuan Jiang ying.ANALYSIS OF THE GENOMIC DNA POLYMORPHISM OF DIFFERENT RACES IN PURE KIND OF CHINESE BEES AND WESTERN BEES[J].Apiculture of China,2001,52(4):12-13. |
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| Authors: | Wang weiping Zhang yajuan Jiang ying |
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| Abstract: | P-8(5'|CCCACCCTTG|3') and W-2(5'|CGGCCCCGGT|3')random primers were used.Th e genomic DNA of different race in pure kind of Chinese bess and western bees we re analysing,which were studied by random amplified polymorphic DNA (RAPD)|poly merase chain reaction (PCR).The results showed that the genomic DNA of different races of Chinese bee as well as western bee were significantly different.It is proved that DNA polymorphic maps in Chinese bee and western bee of different rac es were evidently distinguishable. |
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| Keywords: | Chinese and western bees race of bees genomic DNA polymerase chain reaction |
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