| 捕食线虫性真菌的分离培养技术与形态学 |
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| 引用本文： | 杨晓野,杨莲茹,刘珍莲,杜丽,韩永亭,冯占英.捕食线虫性真菌的分离培养技术与形态学[J].中国兽医学报,2002,22(2):175-177. |
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| 作者姓名： | 杨晓野 杨莲茹 刘珍莲 杜丽 韩永亭 冯占英 |
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| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018
2. 内蒙古呼和浩特市赛罕区农牧局,内蒙古,呼和浩特,010020 |
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| 基金项目： | 国家自然科学基金资助项目 ( 3976 0 0 6 0 ) |
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| 摘 要： | 分离培养捕食线虫性真菌的最佳培养基为 0 .4g/ L 玉米粉琼脂培养基 (CMA) ;0 .4g/ L 玉米粉液体培养基可作为保存菌种培养基。在分离培养过程中 ,必须加入活的线虫第 3期幼虫 ,以诱导菌株产生捕食性器官——菌环、菌网 ,并与其他真菌鉴别。捕食线虫性真菌有菌丝和孢子 2种形态 ,其发育时期不同。本试验分离的少孢节丛孢菌(Arthrobotrys oligospora) ,菌丝发达 ,无色有隔 ,隔距不等 ;分生孢子梗直立不分支 ,分生孢子围绕着分生孢子梗呈轮状排列 ,似梅花瓣状 ,2层以上 ;孢子呈倒卵形 ,丰满无色 ,有横隔 ,游离端大而钝圆 ,梗端小而稍尖。
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| 关 键 词： | 生物防治 线虫 捕食线虫性真菌 分离培养 形态学 |
| 文章编号： | 1005-4545(2002)02-0175-03 |
| 修稿时间： | 2000年12月18 |
Isolating Cultivation and Morphology of Nematode-destroying Fungi |
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| YANG Xiao-ye ,YANG Lian-ru ,LIU Zhen-lian ,DU Li ,HAN Yong-ting ,FENG Zhan-ying.Isolating Cultivation and Morphology of Nematode-destroying Fungi[J].Chinese Journal of Veterinary Science,2002,22(2):175-177. |
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| Authors: | YANG Xiao-ye YANG Lian-ru LIU Zhen-lian DU Li HAN Yong-ting FENG Zhan-ying |
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| Institution: | YANG Xiao-ye 1,YANG Lian-ru 1,LIU Zhen-lian 1,DU Li 1,HAN Yong-ting 1,FENG Zhan-ying 2 |
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| Abstract: | The nematode-destroying fungus had an optimal growth on 0.4 g/L CMA.The liquid medium of same CMA can be used to preserve fungus for a longer time.In order to differentiate the nematode-destroying fungus from other fungi,the alive third larvae of nematode should be given for inducing the fungus to produce trapping organs(rings and networks) when it was isolated.The vegetative hypha and conidia were formed in different development stages.The colourless hypha of Arthrobotrys oligospora developed well in different length of segments and the single conidiophore surrounded by two or more circles of conidia,which look like plum blossoms,stretched up.The conidia are colourless,stout and inverse egg-shaped,and they are divided into two unequal parts:one with a rounder dissociative end is biger and another with a sharper end connected to the conidiophore is smaller. |
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| Keywords: | biological control nematode nematode-destroying fungus isolating cultivation morphology |
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