| Somatic embryogenesis and histological analysis from zygotic embryos in Vitis vinifera L. ＇Moldova＇ |
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| 作者姓名： | LI Hui ;LI Feng-lan ;DU Jing-chuan ;LU Hai ;HE Zheng-quan |
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| 作者单位： | [1]College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China; [2]Biotechnology Research Center, Three Gorges University, Yichang 443000, P. R. China |
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| 基金项目： | supported by “948” Ad-vanced Forestry Technology Introduction Program (Grant No. 2006-4-73). |
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| 摘 要： | We examined the somatic embryogenesis from and histological studies of zygotic embryos of seeds in European Grape ＇Moldova＇ （Vitis vinifera U ＇Moldova＇）. Primary calli were initiated on Nitsch and Nitsch （NN） medium supplemented with 1.0 mg·L^-1 2,4-D and 0.5 mg·L^-1 6-BA. Embryogenic calli were produced upon transfer to a NN medium with 0.5 mg·L^-1 6-BA and 2 mg·L^-1 NAA and somatic embryos were obtained on a half strength MS medium without plant growth regulators. During the somatic embryo germination, an addition of 1.0 mg·L^-1 6-BA in the medium could accelerate somatic embryos to develop into normal plants and increase the conversion rate from 0 to 43.3%. Histological studies of embryogenic calli and somatic embryos demonstrated dynamic changes of proteins and starch grains. The developmental processes of somatic embryos were similar to those of zygotic embryos, including typical epiderma, cotyledon primordium and vascular tissue.
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| 关 键 词： | 林业 失水量 细胞分裂生长 组织学 植物 |
| 收稿时间： | 7 February 2007 |
Somatic embryogenesis and histological analysis from zygotic embryos in <Emphasis Type="Italic">Vitis vinifera</Emphasis> L. ‘Moldova’ |
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| LI Hui,;LI Feng-lan,;DU Jing-chuan,;LU Hai,;HE Zheng-quan.Somatic embryogenesis and histological analysis from zygotic embryos in Vitis vinifera L. ‘Moldova’[J].Forestry Studies in China,2008,10(4):253-258. |
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| Authors: | Hui Li Feng-lan Li Jing-chuan Du Hai Lu Zheng-quan He |
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| Institution: | (1) College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, P. R. China;(2) Biotechnology Research Center, Three Gorges University, Yichang, 443000, P. R. China |
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| Abstract: | We examined the somatic embryogenesis from and histological studies of zygotic embryos of seeds in European Grape ‘Moldova’
(Vitis vinifera L. ‘Moldova’). Primary calli were initiated on Nitsch and Nitsch (NN) medium supplemented with 1.0 mg·L−1 2,4-D and 0.5 mg·L−1 6-BA. Embryogenic calli were produced upon transfer to a NN medium with 0.5 mg·L−1 6-BA and 2 mg·L−1 NAA and somatic embryos were obtained on a half strength MS medium without plant growth regulators. During the somatic embryo
germination, an addition of 1.0 mg·L−1 6-BA in the medium could accelerate somatic embryos to develop into normal plants and increase the conversion rate from 0
to 43.3%. Histological studies of embryogenic calli and somatic embryos demonstrated dynamic changes of proteins and starch
grains. The developmental processes of somatic embryos were similar to those of zygotic embryos, including typical epiderma,
cotyledon primordium and vascular tissue. |
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| Keywords: | zygotic embryo somatic embryo histology Vitis vinifera L |
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