In vitro propagation of Acacia sinuata (Lour.) Merr. via cotyledonary nodes |
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| Authors: | G Vengadesan A Ganapathi R Prem Anand V Ramesh Anbazhagan |
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| Institution: | (1) Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirapalli, Tamilnadu, 620 024, India |
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| Abstract: | Acacia sinuata is a valuable multipurpose tree in Southern India. The tree is over exploited, but its regeneration rate in natural habitat is low. Therefore, it is important to study if it can be regenerated through in vitro micro-propagation. Cotyledonary node and shoot-tip explants excised from 15 day-old in vitro grown seedlings were used to initiate cultures. Maximum number of shoots was induced from cotyledonary node explants on Murashige and Skoog's (MS) medium containing6.66 µM 6-benzylamino purine (BAP) and 4.65µM kinetin (Kn). Subculturing was done in the fresh medium of same composition. The number of shoots formed was comparatively greater in the first subculture. Maximum shoot elongation was achieved (5.5 cm)when subcultured on MS medium supplemented with 1.75 µMgibberellic acid (GA3). In vitro regenerated shoots produced roots when transferred to half strength MS medium supplemented with 7.36 µM indolebutyric acid (IBA). From each cotyledonarynode 30 shoots were obtained within 90 days after two subcultures. The success rate of establishing the rooted plantlets in the field was 55%.This revised version was published online in November 2005 with corrections to the Cover Date. |
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| Keywords: | Leguminous tree Micropropagation Shoot multiplication Stumps |
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