| 野百合试管鳞茎诱导与增殖的研究 |
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| 引用本文： | 刘红美,令狐克勇,方小波.野百合试管鳞茎诱导与增殖的研究[J].农业科学与技术,2008,9(1):18-20. |
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| 作者姓名： | 刘红美 令狐克勇 方小波 |
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| 作者单位： | 贵阳医学院医学生物技术教研室,贵州贵阳550004 |
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| 基金项目： | 贵州省优秀科技教育人才省长基金
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贵州省高层次人才基金
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贵阳医学院校科研和教改项目 |
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| 摘 要： | 利用现代生物技术组织培养方法对野百合进行种质保存和快速繁殖研究，对发展野百合的大规模生产及百合育种工作，保护野生资源和生态环境有重要意义。
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| 关 键 词： | 野百合 试管鳞茎诱导 现代生物技术 繁殖 |
The Study on Induction and Proliferation of Tube Bulbs in Lilium brownii |
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| LIU Hong-mei,LINGHU Ke-yong,FANG Xiao-bo.The Study on Induction and Proliferation of Tube Bulbs in Lilium brownii[J].Agricultural Science ＆ Technology,2008,9(1):18-20. |
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| Authors: | LIU Hong-mei LINGHU Ke-yong FANG Xiao-bo |
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| Institution: | (Medical and Biotechnological Department, Guiyang Medical College, Guiyang, 550004) |
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| Abstract: | With different parts of bulb scale as explants,the proliferation method of C,Guizhou Lilium brownii were studied with 3% sodium hypcohlorite and MS medium with different concentrations of hormones.The results show that it is feasible to disinfect the bulbs of Lilium brownii with 3% sodium hypcohlorite,moreover,the sodium hypochlorite is very cheap and harmless to researchers,experimental materials and environment.MS NAA 0.3 mg/L 6-BA 1.5 mg/L is optimum for the induction of bulbs and,the basal part of Lilium brownie is the optimum explants.After culture for 25 d on the same medium,the tube bulbs could be obtained with the characteristics of high propagation coefficient,strong and new roots.The survival rate is over 90% for transplantufion of tube bulbs with diameter between 1-2 cm.The method developed in the present study can proliferate abundant Lilium brownii seedling in short time. |
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| Keywords: | Lilium brownii Tube bulbs Induction Proliferation |
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