| 1株降酚菌的原生质体制备和再生研究 |
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| 引用本文： | 王垚,曹德菊.1株降酚菌的原生质体制备和再生研究[J].安徽农业科学,2007,35(24):7588-7588,7590. |
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| 作者姓名： | 王垚 曹德菊 |
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| 作者单位： | 安徽农业大学资源与环境学院/安徽省农产品安全重点实验室,安徽合肥,230036 |
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| 基金项目： | 安徽省自然科学基金项目(071105) |
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| 摘 要： | 为了优化组合降酚菌原生质体制备与再生的条件。以从合肥钢厂焦化废水处理池的活性污泥中驯化筛选得到1株高效降酚假单胞菌为材料,通过正交实验,探讨蔗糖浓度、溶菌酶浓度E、DTA浓度和酶解时间对该菌原生质体制备与再生的影响。原生质体制备与再生的最优条件:蔗糖浓度20%,溶菌酶浓度0.5 mg/ml,EDTA浓度0.2%,酶解时间40 min。在此条件下,它的菌株形成率为75.3%,再生率为7.8%。影响降酚假单胞菌原生质体形成率的因素顺序为蔗糖浓度>溶菌酶浓度>作用时间>EDTA浓度。
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| 关 键 词： | 原生质体 降酚菌 再生 正交试验 |
| 文章编号： | 0517-6611（2007）24-07588-01 |
| 修稿时间： | 2007-05-09 |
Study on the Protoplast Preparation and Regeneration of a Strain of Phenol Degrading Bacteria |
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| WAng Yao et al.Study on the Protoplast Preparation and Regeneration of a Strain of Phenol Degrading Bacteria[J].Journal of Anhui Agricultural Sciences,2007,35(24):7588-7588,7590. |
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| Authors: | WAng Yao |
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| Institution: | College of Resource and Environment, Anhui Agricultural University, Hefei,Anhui 230036 |
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| Abstract: | The aim was to optimize the protoplast preparation and regeneration conditions of phenol degrading bacteria.With a high-efficiency phenol degrading pseudomonad strain obtained through domestication and screening from active sewage in coking wastewater treatment pool of Hefei Steel Mill as materials,the effects of 4 factors as sucrose concentration,lysozyme concentration,EDTA concentration and the time of enzyme hydrolysis on the protoplast preparation and regeneration of the bacteria was discussed through the orthogonal experiment.The optimum conditions of protoplast preparation and regeneration were as follows: sucrose concentration of 20%,lysozyme concentration of 0.5 mg/ml,EDTA concentration of 0.2% and the time of enzyme hydrolysis of 40 min.Under these conditions,its strain formation rate was 75.3% and regeneration rate was 7.8%.The sequence of 4 factors influencing protoplast formation rate of phenol degrading pseudomonad was sucrose concentration> lysozyme concentration>acting time>EDTA concentration. |
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| Keywords: | Protoplast Phenol degrading bacteria Regeneration Orthogonal experiment |
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