| 丙烷脒对灰霉病菌菌丝形态和超微结构的影响 |
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| 引用本文： | 陈安良,冯美杰,冯俊涛,何军,张兴.丙烷脒对灰霉病菌菌丝形态和超微结构的影响[J].中国农业科学,2007,40(3):633-637. |
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| 作者姓名： | 陈安良 冯美杰 冯俊涛 何军 张兴 |
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| 作者单位： | 1. 西北农林科技大学无公害农药研究服务中心,杨凌,712100;陕西省生物农药工程技术研究中心,杨凌,712100
2. 西北农林科技大学无公害农药研究服务中心,杨凌,712100 |
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| 基金项目： | 国家科技攻关计划;西北农林科技大学校科研和教改项目 |
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| 摘 要： | 【目的】杀菌剂对病菌菌丝形态和超微结构的影响，是进行杀菌剂作用机理研究的基础。【方法】采用扫描电镜和透射电镜，研究了丙烷脒对灰霉病菌菌丝形态和超微结构的影响。【结果】药剂处理1 d后，菌丝局部不规则膨胀，菌丝隔膜间距变短；药剂处理3 d，菌丝局部严重膨大，且膨胀部位失水畸形。透射电镜观察表明，药剂处理1 d后，菌丝细胞线粒体体积增大、数目增多，细胞核的结构已不明显，细胞内出现囊泡；3 d后，各细胞器结构紊乱，并含有大量的无膜透明内含物，有较大的空腔形成，细胞壁外围沉积大量的外渗物，菌丝组织坏死。【结论】丙烷脒对灰霉病菌菌丝形态和超微结构的影响不同于常用杀菌剂品种，预示丙烷脒的作用机理独特。对丙烷脒作用机理进行深入研究，有望发现杀菌剂新的作用靶标，为杀菌剂新品种的开发提供依据。
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| 关 键 词： | 丙烷脒 灰霉病菌 超微结构 |
| 收稿时间： | 2005-3-10 |
| 修稿时间： | 2005-03-07 |
Effects of Propamidine on Mycelial Morphology and Ultrastructure of Botrytis cirerea |
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| CHEN An-liang,FENG Mei-jie,FENG Jun-tao,HE Jun,ZHANG Xing.Effects of Propamidine on Mycelial Morphology and Ultrastructure of Botrytis cirerea[J].Scientia Agricultura Sinica,2007,40(3):633-637. |
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| Authors: | CHEN An-liang FENG Mei-jie FENG Jun-tao HE Jun ZHANG Xing |
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| Institution: | 1.Biorational Pesticide Research and Development Center, Northwest Agriculture and Forestry University, Yangling 712100; 2.Shaanxi Research Center of Biopesticide Engineering and Technology, YangLing 712100 |
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| Abstract: | Objective ] The research on the effects of fungicide on morphology and ultrastructure of fungi mycelia cell was the basic of antifungi mechanism. Method] The effects of propamidine, a novel fungicide, on morphology and ultrastructure of mycelial cell ofBotrytis cirerea were investigated under light scanning and transmission electronic microscopy. Result]The studies revealed that propamidine caused a series of marked morphology and structural alterations of hyphae. Treated with propamidine after one day, the space between mycelium septum was shortened, the part of mycelium swelled, and the volume of mycelium cell mitochondrion was swollen. Its number increased, and the cell nuclei also displayed an alteration. After three days, the swelled part of mycelium was malformed and the configuration of mycelium cell was destroyed. Conclusion] Compared with other fungicides, the changes of morphology and ultrastructures of mycelial cell induced by propamidine were unique. Therefore the antifungi mechanism of propamidine was worthy of studying. |
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| Keywords: | Propamidine Botrytis cirerea Ultrastructure |
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