| MS培养基中氮、磷无机盐对鳗草克隆幼苗生存的影响 |
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| 引用本文： | 张飞,郑凤英.MS培养基中氮、磷无机盐对鳗草克隆幼苗生存的影响[J].农学学报,2020,10(8):57-62. |
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| 作者姓名： | 张飞 郑凤英 |
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| 作者单位： | 山东大学（威海）海洋学院,山东威海 264209 |
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| 基金项目： | 2016威海市大学共建项目“刺参天然养殖海域大叶藻和红纤维虾海藻混合海草场重建技术的研究”(2016-1) |
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| 摘 要： | 为了探究MS培养基中氮、磷无机营养盐对鳗草生长的影响。单独添加不同浓度KNO3、NH4NO3和KH2PO4的天然海水中培养鳗草克隆苗,于7、14天进行植株形态学观察和叶片叶绿素含量测定。其中,KNO3的添加量为MS培养基中的1/2倍、1倍和2倍及NO3-总浓度为39.4 mmol/L,KH2PO4和NH4NO3的添加量均为MS培养基中各自浓度的1/2、1和2倍。结果表明,叶绿素含量以KNO3处理组最高(P<0.05),克隆苗培养14天无毒害现象,且有新叶发出;NH4NO3和KH2PO4处理组均对克隆苗有明显毒害作用,NH4NO3处理组叶片48 h后开始褐化,7天后茎尖、根尖明显变软并褐化,14天后植株死亡;KH2PO4处理组培养14天后叶片平均褐化率达56%。因此,1/2、MS培养基均不适用于鳗草的室内培养,其培养基的氮源应为单独添加的NO3--N或高浓度NO3--N+低浓度NH4+-N。
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| 关 键 词： | 鳗草 克隆幼苗 MS培养基 氮无机盐 磷无机盐 室内培养 |
| 收稿时间： | 2019-05-24 |
Nitrogen and Phosphorus Inorganic Salts in MS Medium: Effects on Growth and Survival of Zostera marina Developing Ramets |
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| Zhang Fei,Zheng Fengying.Nitrogen and Phosphorus Inorganic Salts in MS Medium: Effects on Growth and Survival of Zostera marina Developing Ramets[J].Journal of Agriculture,2020,10(8):57-62. |
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| Authors: | Zhang Fei Zheng Fengying |
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| Institution: | Marine College, Shandong University (Weihai), Weihai 264209, Shandong, China |
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| Abstract: | To explore the effects of nitrogen and phosphorus inorganic salts in MS medium on the growth of Zostera marina, the young ramets of Z.marina were cultured in natural seawater with different concentrations of KNO3, NH4NO3 and KH2PO4 , respectively, and the morphological observation was conducted and leaf chlorophyll content was determined at 7 and 14 days; among them, the addition of KNO3 was 1/2, 1 and 2 times as much as that of MS medium, and the total concentration of NO3- was 39.4 mmol/L, while the addition of KH2PO4 and NH4NO3 was 1/2, 1 and 2 times as much as that of MS medium respectively. The results showed that: the chlorophyll content in KNO3 treatment group was the highest (P<0.05), the developing ramets were cultured for 14 days without toxic phenomenon and new leaves sprouted; however, the NH4NO3 and KH2PO4 treatment groups both had obviously toxic effects on the ramets; the leaves of ramets began to brown after culturing for 48 h in NH4NO3 treatment group; subsequently, the tips of root and rhizome softened and began to brown after 7 days of incubation and finally the ramets’ shoots died after 14 days; while the ramets’ leaves of KH2PO4 treatment group had the average browning rate up to 56% after culturing 14 days. Thus, neither 1/2 MS medium nor MS medium is suitable for in vitro culture of Z.marina, and the nitrogen source of the culture medium is suggested as addition of NO3--N alone or a high concentration NO3--N with a low concentration of NH4+-N. |
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| Keywords: | Zostera marina Developing Ramets MS Medium Nitrogen Inorganic Salt Phosphorus Inorganic Salt Indoor Culture |
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