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| β-肾上腺素受体激动剂莱克多巴胺兔单克隆抗体的制备(英文) | |
| 引用本文： | 张建群,杨扬,王艺熹,张铭,裘霁,郑晓冬.β-肾上腺素受体激动剂莱克多巴胺兔单克隆抗体的制备(英文)[J].农业生物技术学报,2007,15(3):398-403. |
| 作者姓名： | 张建群 杨扬 王艺熹 张铭 裘霁 郑晓冬 |
| 作者单位： | 1. 浙江大学生物系统工程与食品科学学院,杭州,310029;浙江嘉兴职业技术学院,嘉兴,314036 2. 浙江大学生物系统工程与食品科学学院,杭州,310029 3. 浙江大学生命科学学院 4. 杭州宜康生物技术有限公司,杭州,310015 |
| 摘 要： | 本文制备了一种针对β-肾上腺素受体激动剂莱克多巴胺（Ractopamine）的兔单克隆抗体。以BSA和OVA为载体蛋白，合成的莱克多巴胺-BSA作为免疫抗原。选用WHB黑眼大白兔为免疫动物，将免疫的兔脾细胞和240E骨髓瘤细胞杂交获得杂交瘤细胞。细胞株A3-2-6产生的上清被用于进一步的免疫分析，其IC50值为4.09ng/ml，线性范围在0.1～100ng/ml之间，与莱克多巴胺具有类似结构的β-肾上腺素受体激动剂如克伦特罗，肾上腺素，去甲肾上腺素，苯氧丙酚胺，沙丁胺醇和多巴胺都没有交叉反应性。总之，本实验获得了一种较高特异性的莱克多巴胺兔单克隆抗体。 |
| 关 键 词： | 兔单克隆抗体 β-肾上腺素受体激动剂 莱克多巴胺 ELISA |
| 文章编号： | 1006-1304（2007）03-0398-06 |
| 收稿时间： | 2006-04-04 |
| 修稿时间： | 2006-04-042006-05-30 |
Production of a Rabbit Monoclonal Antibody for β-adrenergic Agonist Ractopamine Detection |
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| ZHANG Jian-qun,YANG Yang,WANG Yi-xi,ZHANG Ming,QIU Ji,ZHENG Xiao-dong.Production of a Rabbit Monoclonal Antibody for β-adrenergic Agonist Ractopamine Detection[J].Journal of Agricultural Biotechnology,2007,15(3):398-403. | |
| Authors: | ZHANG Jian-qun YANG Yang WANG Yi-xi ZHANG Ming QIU Ji ZHENG Xiao-dong |
| Institution: | 1.Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China; 2.Jiaxing Vocational Technology College, Jiaxing 314036, China; 3. College of Life Science, Zhejiang University, Hangzhou 310029, China; 4.Hangzhou Epitomics Biotechnology Co. Ltd, Hangzhou 310015, China |
| Abstract: | A rabbit monoclonal antibody(RabMab) was generated toward theβ-Adrenergic agonist Ractopamine. Bovine serum albumin(BSA) and ovalbumin(OVA) were used as carrier proteins and Ractopamine-glutarate- BSA was used as the antigen. The WHB dark-eye rabbits were used as antibody generation animals. B lymphocytes from immuned rabbits and 240E myeloma cells were fused to generate hybridoma cells. Supernatant from clone A3-2-6 was used for the development of an immunoassay. The selected antibody was specific for ractopamine with an IC50 of 4.09ng/ml. The linear range of the inhibition rate vs log ractopamine competition curve generated in PBST was 0.1～100ng/ml. β-adrenergic agonists which had similar structures to ractopamine such as clenbuterol hydrochloride, isoxsuprine, epinephrine, norepinephrine, isoprenaline, salbutamol and dopamine showed no cross-reactivity. In conclusion, a highly specific RabMab to ractopamine hydrochloride was developed that could be used in indirect competitive ELISA screening assays. |
| Keywords: | ELISA |
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