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| 产油微生物的筛选 | |
| 引用本文： | 叶思特,郭丽琼,刘晓蓉,陈晓阳,林俊芳.产油微生物的筛选[J].华南农业大学学报,2012,33(3):384-387. |
| 作者姓名： | 叶思特 郭丽琼 刘晓蓉 陈晓阳 林俊芳 |
| 作者单位： | 1. 华南农业大学食品学院,广东广州,510642 2. 华南农业大学食品学院,广东广州510642;华南农业大学生物质能研究所,广东广州510642 3. 华南农业大学生物质能研究所,广东广州,510642 |
| 基金项目： | 广东省科技攻关项目，华南农业大学"211工程"三期项目资助 |
| 摘 要： | 采用苏丹黑B染色法、甲醇-氯仿法,从30份土壤样品中筛选到9株产油霉菌和4株产油酵母.经5.8S rDNA序列分析,9株产油霉菌分别为蓝状菌Talaromyces trachyspermus、嗜松青霉Penicillium pinophillum、青霉Pe.sp.、微紫青霉Pe.janthinellum、淡紫拟青霉Paecilomyces lilacinus、简青霉Pe.simplicissimum.、棘孢曲霉Aspergillus aculea-tus、木霉Trichoderma sp.、拟康宁木霉Tr.koningiopsis,4株产油酵母分别是毕赤酵母Pichia caribbica、假丝孢酵母Candida sp.、季也蒙酵母Meyerozyma guilliermondii和季也蒙毕赤酵母Pi.guilliermondii.通过摇瓶培养,测定了各菌株的产油量,结果表明:霉菌产油量最高的是嗜松青霉,产油量为26.4%;酵母产油量最高的是假丝孢酵母,产油量为44.3%.提取毕赤酵母的油脂,采用气相色谱-质谱联用分析,测得其油脂成分主要是14～20碳的短链脂肪酸. |
| 关 键 词： | 产油微生物 筛选 微生物油脂 |
| 收稿时间： | 2011/8/29 0:00:00 |
Screening of the Oleaginous Microorganisms |
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| YE Si-te,GUO Li-qiong,LIU Xiao-rong,CHEN Xiao-yang and LIN Jun-fang.Screening of the Oleaginous Microorganisms[J].Journal of South China Agricultural University,2012,33(3):384-387. | |
| Authors: | YE Si-te GUO Li-qiong LIU Xiao-rong CHEN Xiao-yang and LIN Jun-fang |
| Institution: | 1,2(1 College of Food Science,South China Agricultural University,Guangzhou 510642,China; 2 Institute of Biomass Energy,South China Agricultural University,Guangzhou 510642,China) |
| Abstract: | Nine oleaginous moulds and four oleaginous yeasts were isolated from 30 soil samples by using Sudan Black B staining and methanol-chloroform extraction.These oleaginous microorganisms were identified by 5.8S rDNA sequence analysis.Nine oleaginous moulds were assigned to Talaromyces trachyspermus,Penicillium pinophillum,Pe.sp.,Pe.janthinellum,Paecilomyces lilacinus,Pe.simplicissimum,Aspergillus aculeatus,Trichoderma sp.and Tr.koningiopsis.Four oleaginous yeasts were Pichia caribbica,Candida sp.,Meyerozyma guilliermondii and Pi.guilliermondii.The lipid yields of the oleaginous microorganisms were tested by cultivation in shake flasks.The highest lipid yield was up to 26.4% by P.pinophillum among 9 oleaginous moulds and 44% by Candida sp.among 4 oleaginous yeasts.The lipid extracted from Candida sp.was mainly composed of 14~20 short chain fatty acids by GC-MS analysis. |
| Keywords: | oleaginous microorganisms screening microbial lipids |
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