| 抑菌植物的筛选及甘草对5种植物病原真菌抑菌活性的研究 |
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| 引用本文： | 罗建军,翁群芳,胡美英.抑菌植物的筛选及甘草对5种植物病原真菌抑菌活性的研究[J].华南农业大学学报,2012,33(3):356-360. |
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| 作者姓名： | 罗建军 翁群芳 胡美英 |
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| 作者单位： | 华南农业大学资源环境学院,广东广州,510642 |
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| 摘 要： | 为了寻找新的植物源杀菌剂,采用菌丝生长速率法测定了17种植物提取物对荔枝霜疫霉病菌Peronophythora litchi的抑菌活性.结果显示:辣蓼Polygonum lapathifolium、白花油茶Camellia oleifera甲醇提取物(干粉)10 mg/mL处理7 d后的抑菌率分别达74.37%、77.02%,而甘草Glycyrrhiza uralensis根甲醇提取物相同质量浓度时的抑菌率达到97.83%;甲醇对甘草的提取率为23.04%,甘草的甲醇提取物1和5 mg/mL处理对荔枝霜疫霉病菌菌丝生长的抑菌率分别为63.84%和80.68%,对荔枝霜疫霉病菌孢子囊芽管萌发的抑制中质量浓度(EC50)为0.47 mg/mL,对荔枝霜疫霉病菌、辣椒疫霉病菌Phytophthora capsici、芒果炭疽病菌Colletotrichum gloeosporioides、香蕉枯萎病菌Fusarium oxysporum f.sp.cubense和草莓叶斑病菌Cladosporium herbarum菌丝生长的EC50依次为0.62、0.89、9.70、6.42和4.53 mg/mL,表明甘草甲醇提取物具有较好的抑菌活性,有望继续研究开发成新的杀菌剂.
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| 关 键 词： | 提取物 甘草 抑菌活性 |
| 收稿时间： | 5/8/2012 12:00:00 AM |
Studies on Screening of Antifungal Plants and Antifungal Activities of Glycyrrhiza uralensis Against Five Phytopathogens |
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| LUO Jian-jun,WENG Qun-fang and HU Mei-ying.Studies on Screening of Antifungal Plants and Antifungal Activities of Glycyrrhiza uralensis Against Five Phytopathogens[J].Journal of South China Agricultural University,2012,33(3):356-360. |
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| Authors: | LUO Jian-jun WENG Qun-fang and HU Mei-ying |
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| Institution: | (College of Natural Resources and Environment,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | In order to find new fungicides,antifungal activities of 17 plant extracts against Peronophythora litchi were determined by using mycelial growth rate method.The main results were as follows: after 7 days’ treatment with a dry-powder mass concentration of 10 mg/mL,the methanol extracts of Polygonum lapathifolium and Camellia oleifera showed inhibition rates of 74.37% and 77.02%,respectively,while the methanol extract of Glycyrrhiza uralensis(root) showed inhibition rates of 97.83% at the same test mass concentration;extraction ratio reached 20.80% by using methanol to extract Glycyrrhiza uralensis(root) and it showed inhibition rates of 63.84% and 80.68% on P.litchi with dry-powder mass concentrations of 1.0 and 5.0 mg/mL,respectively,and the median inhibitory mass concentration was 0.47 mg/mL against the germination of sporangia of P.litchi.The median inhibitory mass concentration against P.litchi,Phytophthora capsici,Colletotrichum gloeosporioides,Fusarium oxysporum f.sp.cubense,and Cladosporium herbarum was 0.62,0.89,9.70,6.42 and 4.53 mg/mL,respectively.These results suggest that the methanol extract of G.uralensis(root) has good antifungal activity,and it is expected to develop into a new fungicide with further research. |
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| Keywords: | plant extract Glycyrrhiza uralensis antifungal activity |
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