| 水仙鳞茎中粗多糖的提取工艺优化 |
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| 引用本文： | 牛炜,潘东明,贾媛颖.水仙鳞茎中粗多糖的提取工艺优化[J].北方园艺,2012(10):64-66. |
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| 作者姓名： | 牛炜 潘东明 贾媛颖 |
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| 作者单位： | 福建农林大学园艺学院,福建福州350002;园艺产品贮运保鲜研究所,福建福州350002;福建农林大学园艺学院,福建福州,350002 |
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| 基金项目： | 国家科技支撑计划资助项目 |
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| 摘 要： | 为初步确定水仙鳞茎中的粗多糖含量,采用热水浸提法提取水仙鳞茎中的粗多糖。同时以粗多糖得率为评价指标,以提取温度(℃)、液固比(mL/g)、乙醇体积比(无水乙醇mL/提取液mL)、提取时间(h)为因素优化热水浸提工艺。结果表明:最佳提取工艺为提取时间5.5h,提取温度100℃,液固比10mL/g,乙醇加量比5(醇沉时加入无水乙醇量mL/样品溶液量mL)。其中提取温度是影响水仙鳞茎粗多糖得率的最主要因素。最优条件下提取粗多糖得率6.51445%,水仙鳞茎粗多糖含量为3.8130%。
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| 关 键 词： | 水仙鳞茎粗多糖 热水浸提 正交实验 |
Optimization of the Extraction of Crude Polysaccharides from Bulb of Narcissus |
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| NIU Wei
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PAN Dong-ming
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JIA Yuan-ying.Optimization of the Extraction of Crude Polysaccharides from Bulb of Narcissus[J].Northern Horticulture,2012(10):64-66. |
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| Authors: | NIU Wei PAN Dong-ming JIA Yuan-ying |
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| Institution: | 1(1.College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002;2.Fuzhou Horticultural Products Ship and Preservation Institute,Fuzhou,Fujian 350002) |
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| Abstract: | To measure the amount of the crude polysaccharides from the bulb of Narcissus(CPNs),water extraction technology were used to get CPNs.Optimizing the extraction method with the extraction ratio as index,and used the liquid-solid ratio,extraction temperature,ethanol-sample ratio and extraction time as factors were studied.The results showed that the best extraction method was extracting 5.5 h,at 100℃,using 10 as liquid-solid ratio and setting the ethanol-sample ratio 5,and the extraction ratio of CNPs was 6.51445%,the amount of CPNs was 3.8130%. |
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| Keywords: | crude polysaccharides from the bulb of Narcissus(CPNs) water extraction orthogonal test |
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