| 台尔曼忍冬组织培养技术工厂化繁育研究 |
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| 引用本文： | 张慧洁,王立英,袁婷,孙慧慧.台尔曼忍冬组织培养技术工厂化繁育研究[J].北方园艺,2012(8):131-133. |
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| 作者姓名： | 张慧洁 王立英 袁婷 孙慧慧 |
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| 作者单位： | 1. 北京森淼种业有限公司,北京,102211
2. 北京森淼种业有限公司,北京 102211;宁夏森淼种业生物工程有限公司,宁夏 银川 750004 |
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| 基金项目： | 北京市科技计划资助项目 |
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| 摘 要： | 以台尔曼忍冬为试材进行织培养工厂化繁育。结果表明:台尔曼忍冬适宜的分化培养基配方为MS+0.6mg/L 6-BA+0.1mg/L NAA+100mg/L水解乳蛋白,分化系数为3.2;筛选出适宜的生根培养基配方为1/2MS+0.6mg/L 6-BA+0.1mg/L NAA,生根率92%;练苗5～7d后,在草炭∶蛭石∶珍珠岩=2∶1∶1的基质中移栽,成活率90%;大田定植成活率为95%。该研究建立了台尔曼忍冬组织培养技术工厂化繁育体系,达到了高效快速,且再生繁育体系稳定性好,可为今后的台尔曼忍冬组织培养繁殖技术提供参考。
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| 关 键 词： | 台尔曼忍冬 分化系数 移栽成活率 |
Research on Factory Breeding Tissue Culture Technology of Lonicera × tellmanniana Spaeth |
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| ZHANG Hui-jie
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WANG Li-ying
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YUAN Ting
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SUN Hui-hui.Research on Factory Breeding Tissue Culture Technology of Lonicera × tellmanniana Spaeth[J].Northern Horticulture,2012(8):131-133. |
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| Authors: | ZHANG Hui-jie WANG Li-ying YUAN Ting SUN Hui-hui |
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| Institution: | 1(1.Beijing Senmiao(Ocean Green) Seedling Limited Company,Beijing 102211;2.Ningxia Senmiao(Ocean Green) Biological Engineering Limited Company,Yinchuan,Ningxia 750004) |
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| Abstract: | With Lonicera×tellmannianaSpaeth as test material,factory breeding tissue culture technology were studied.The results showed that appropriate differentiation medium of Lonicera×tellmannianaSpaeth was MS+0.6mg/L 6-BA +0.1mg/L NAA+100mg/L hydrolyzed milk protein the coefficient of differentiation was 3.2;the best formula for the rooting medium was 1/2MS+0.6mg/L 6-BA+0.1mg/L NAA,the rate of rooting was 92%;after acclimatized for 5～ 7d,the rate of survival was 90%,when the regeneration plantlets were transplanted to substate containing peat∶ vermiculite∶perlite(2∶1∶1).The industrialization breed rate by tissue culture technology of Lonicera×tellmanniana Spaeth established through this study was rapid,the stability of regeneration system was well,could provide reference for the future tissue culture propagation technology of the Lonicera×tellmannianaSpaeth. |
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| Keywords: | Lonicera×tellmannianaSpaeth coefficient of differentiation survival rate of transplanting |
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