| CsFAD7基因转化烟草的研究 |
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| 引用本文： | 刘春香,冷传远,刘福.CsFAD7基因转化烟草的研究[J].北方园艺,2012(11):120-123. |
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| 作者姓名： | 刘春香 冷传远 刘福 |
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| 作者单位： | 潍坊学院山东省高校生物化学与分子生物学重点实验室,山东潍坊,261061;潍坊学院山东省高校生物化学与分子生物学重点实验室,山东潍坊,261061;潍坊学院山东省高校生物化学与分子生物学重点实验室,山东潍坊,261061 |
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| 基金项目： | 山东省自然科学基金资助项目 |
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| 摘 要： | 以"革新一号"烟草种子为试材,采用根癌农杆菌介导的烟草叶盘转化法将来源于黄瓜的脂肪酸去饱和酶基因(FAD7)转入烟草,并在附加300mg/L氨苄青霉素(Amp)的MS+0.1mg/L NAA+1.0mg/L 6-BA的培养基上诱导植株分化,再生芽在附加300mg/L Amp和100mg/L卡那霉素(Kan)的MS培养基上生根,获得再生植株。结果表明:Kan阳性植株经PCR检测筛选,得到转基因阳性植株,证明异源FAD基因已转入烟草基因组中。T1代经PCR-South-ern检测证明转化基因可以遗传;转基因烟草在低温胁迫下生长较好,叶绿素荧光参数F0值下降不大,Fv/Fm值保持较高,表现了对低温的耐受能力,脂肪酸分析表明,该基因可以使烟草叶片的亚麻酸含量比例升高。
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| 关 键 词： | 烟草 根癌农杆菌 黄瓜 FAD7基因 |
Study on the Transformation of Cs-FAD7 Gene into Nicotiana tabacum |
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| LIU Chun-xiang
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LENG Chuan-yuan
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LIU Fu-yan.Study on the Transformation of Cs-FAD7 Gene into Nicotiana tabacum[J].Northern Horticulture,2012(11):120-123. |
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| Authors: | LIU Chun-xiang LENG Chuan-yuan LIU Fu-yan |
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| Institution: | -yan(Weifang University,Key Laboratory of Biochemistry and Molecular Biology of University in Shandong Province,Weifang,Shandong 261061) |
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| Abstract: | Using ’Gexin No.1’ tobacco seeds as test materials,FAD7 gene was transferred into tobacco leaves with the help of Agrobacterium tumefaciens.Shoots were regenerated on MS medium supplemented with 300 mg/L Ampicillin,1.0 mg/L 6-BA and 0.1 mg/L NAA and rooted on MS medium with 300 mg/L Ampicillin and 100 mg/L Kanamycin.PCR analysis proved FAD7 gene integration into Kanamycin resistance plants.The results of PCR analysis in T1 generation transgenic plants showed that the transformed gene could inherit to next generation,it grew well under low temperature.The parameters of leave chlorophyll fluorescence F0 decreased slightly,and the values of Fv/Fm kept high level after CsFAD7 gene transformed into tobacco.fatty acid analysis showed that the contents ratio of linolenic acid in leaves were higher in transgenic tobacco than in wild type tobacco. |
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| Keywords: | Nicotiana tabacum Agrobacterium tumefaciens cucumber FAD 7 gene |
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