|
|||||
|
|
| 胡萝卜愈伤组织诱导培养的研究 | |
| 引用本文： | 汪洪,夏鸿亮,李校堃,杨树林.胡萝卜愈伤组织诱导培养的研究[J].北方园艺,2012(6):103-105. |
| 作者姓名： | 汪洪 夏鸿亮 李校堃 杨树林 |
| 作者单位： | 1. 南京理工大学环境与生物工程学院,江苏南京210094;温州医学院药学院,浙江温州325035 2. 温州医学院药学院,浙江温州,325035 3. 南京理工大学环境与生物工程学院,江苏南京,210094 |
| 基金项目： | 浙江省自然科学基金资助项目(Y3100459) |
| 摘 要： | 以胡萝卜"七寸人参"为试材,探讨了不同外植体、激素、培养基、光照对胡萝卜愈伤组织诱导的影响。结果表明:胡萝卜愈伤组织的诱导以下胚轴为外植体为宜;在添加2,4-D1.0mg/L和KT 0.5mg/L的MS培养基上愈伤组织的诱导频率为100%,且愈伤组织生长良好,增殖快速;光照对愈伤组织的诱导没有影响,但却进一步阻碍了愈伤组织的增殖生长。 |
| 关 键 词： | 胡萝卜 愈伤组织 组织培养 |
A Study on Calli Induction and Culture from Daucus carota L |
|
| WANG Hong , XIA Hong-liang , LI Xiao-kun , YANG Shu-lin.A Study on Calli Induction and Culture from Daucus carota L[J].Northern Horticulture,2012(6):103-105. | |
| Authors: | WANG Hong XIA Hong-liang LI Xiao-kun YANG Shu-lin |
| Institution: | 1 (1.School of Environmental and Biological Engineering,Nanjing University of Science and Technology,Nanjing,Jiangsu 210094;2.School of Pharmacy,Wenzhou Medical College,Wenzhou,Zhejiang 325035) |
| Abstract: | Calli induction and proliferation from explants were regulated by several factors,the effect of hormones,different explants,light and media on calli induction from Daucus carota L.was investigated.The results indicated that the optimal explant for calli induction from carrot was hypocotyl rather than cotyledon.The optimal medium for calli induction was MS agar medium supplemented with 3% sucrose,2,4-D 1.0 mg/L and KT 0.5 mg/L.The induced calli frequency of the carrot hypocotyls on this medium was 100%.Light had no effect on calli induction but it influenced calli proliferation. |
| Keywords: | carrot calli tissue culture |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |