| 膜荚黄芪子叶节植株再生体系的建立 |
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| 引用本文： | 王树斌,全雪丽,周玉红,吴松权.膜荚黄芪子叶节植株再生体系的建立[J].北方园艺,2012(6):177-178. |
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| 作者姓名： | 王树斌 全雪丽 周玉红 吴松权 |
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| 作者单位： | 延边大学农学院,吉林延吉,133002;延边大学农学院,吉林延吉,133002;延边大学农学院,吉林延吉,133002;延边大学农学院,吉林延吉,133002 |
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| 基金项目： | 国家自然科学基金资助项目(30860036);吉林省自然科学基金资助项目(201115228) |
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| 摘 要： | 以膜荚黄芪无菌苗子叶节为外植体,建立了简单、高效和稳定的膜荚黄芪再生体系。结果表明:不定芽诱导的最适培养基是MS+6-BA 1.0mg/L+NAA 0.10mg/L,每个子叶节外植体平均出芽数为3个以上,再生频率达75.5%;生根最适培养基为1/2MS+IBA 0.5mg/L;移栽后成活率达75%以上。
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| 关 键 词： | 膜荚黄芪 子叶节 再生体系 |
Establishment of Plantlet Regeneration System of Cotyledon Node for Astragalus membranaceus |
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| WANG Shu-bin
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QUAN Xue-li
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ZHOU Yu-hong
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WU Song-quan.Establishment of Plantlet Regeneration System of Cotyledon Node for Astragalus membranaceus[J].Northern Horticulture,2012(6):177-178. |
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| Authors: | WANG Shu-bin QUAN Xue-li ZHOU Yu-hong WU Song-quan |
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| Institution: | (College of Agriculture,Yanbian University,Yanji,Jilin 133002) |
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| Abstract: | The cotyledon node obtained from aseptic seedling of Astragalus membranaceus were used as the explant and a simple,stable and high efficient regeneration system for A.membranaceus were established.The results showed that the optimum medium for adventitious shoot regeneration was MS+6-BA 1.0 mg/L+NAA 0.10 mg/L,the average number of differential buds per cotyledon node was above 3,and the regenerated frequency was 75.5%.The suitable rooting medium was 1/2MS+IBA 0.5 mg/L and the survive rate was above 75% after transplantation. |
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| Keywords: | Astragalus membranaceus cotyledon node regeneration system |
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