| 影响红枣幼茎组培增殖因子的研究 |
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| 引用本文： | 杨伟,杨宇,徐崇志,高疆生.影响红枣幼茎组培增殖因子的研究[J].北方园艺,2012(11):116-119. |
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| 作者姓名： | 杨伟 杨宇 徐崇志 高疆生 |
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| 作者单位： | 塔里木大学植物科学学院,新疆阿拉尔,843300;新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔,843300;塔里木大学植物科学学院,新疆阿拉尔843300;新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300 |
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| 基金项目： | "973"计划前期研究专项资助项目 |
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| 摘 要： | 以灰枣、骏枣的幼嫩茎段为外植体,研究了不同有机添加物对红枣组培继代增殖的影响。结果表明:外植体灭菌采用酒精处理20s、升汞处理10min,茎段污染率低,平均出芽数比较高;灰枣初代培养基:MS+6-BA 1.0mg/L+NAA 0.2mg/L,骏枣初代培养基:MS+6-BA4.0mg/L+NAA 0.5mg/L;灰枣继代培养基:MS+6-BA 2.0mg/L+NAA 0.4mg/L,骏枣继代培养基:MS+6-BA 4.0mg/L+NAA 0.5mg/L;最适有机添加物为水解酪蛋白,在灰枣和骏枣的继代培养中的添加浓度分别为400mg/L和500mg/L。
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| 关 键 词： | 灰枣 骏枣 组织培养 有机添加物 继代培养 |
Study on the Factor Influencing the Propagation to the Soft-Stem Tissue Culture of the Jujube |
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| YANG Wei
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YANG Yu
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XU Chong-zhi
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GAO Jiang-sheng.Study on the Factor Influencing the Propagation to the Soft-Stem Tissue Culture of the Jujube[J].Northern Horticulture,2012(11):116-119. |
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| Authors: | YANG Wei YANG Yu XU Chong-zhi GAO Jiang-sheng |
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| Institution: | 1,2 (1.College of Plant Science,Tarim University,Alar,Xinjiang 843300;2.Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin,Xinjiang Production and Construction Group,Alar,Xinjiang 843300) |
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| Abstract: | Using soft stems of ’Huizao’ jujube and ’Junzao’ jujube as explants,the effects of tissue culture mulitiplication on jujube with different organic additive were studied.The results showed that the treating by alcohol mercuric chloride for 20 s and 10 min,the effect of explant sterilization was better,the pollution percent of jujuba stem segments was low and the average budding rate was higher.The primary media for ’Huizao’ jujube were MS+6-BA 1.0 mg/L+NAA 0.2 mg/L and the primany media for ’Junzao’ jujube were MS+6-BA 4.0 mg/L+NAA 0.5 mg/L;The subculture media for ’Huizao jujube’ were MS+6-BA 2.0 mg/L+NAA 0.4 mg/L and for ’Junzao’ jujube were MS+6-BA 4.0 mg/L+NAA 0.5 mg/L.The proper organic additives was hydrolytic castein,which additive concentration in ’Junzao’ and ’Huiza’ jujube were 400 mg/L and 500 mg/L. |
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| Keywords: | ’Huizao’ jujube ’Junzao’ jujube tissue culture organic material subcuhure |
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