| 软儿梨果肉中黄酮类化合物的提取及含量测定 |
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| 引用本文： | 查慧领,石玉平,任世霞.软儿梨果肉中黄酮类化合物的提取及含量测定[J].北方园艺,2012(10):42-44. |
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| 作者姓名： | 查慧领 石玉平 任世霞 |
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| 作者单位： | 青海卫生职业技术学院,青海西宁,810000;青海师范大学化学系,青海西宁,810008;青海油田钻采工艺研究院,甘肃敦煌,736200 |
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| 摘 要： | 以青海产软儿梨为试材,以乙醇为提取剂,通过单因素、正交实验对黄酮类化合物进行提取,研究其最佳提取条件。结果表明:提取的最佳条件为乙醇浓度95%,浸提温度为80℃,乙醇加入量为90mL,浸提时间为3.0h。以芦丁为标准物,通过分光光度法测定软儿梨果肉中总黄酮的含量为0.0189g/100g。
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| 关 键 词： | 软儿梨 黄酮类化合物 单因素试验 正交实验 提取 |
Determination of Flavonoids Extraction in Soft Pear from Qinghai Province |
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| CHA Hui-ling
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SHI Yu-ping
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REN Shi-xia.Determination of Flavonoids Extraction in Soft Pear from Qinghai Province[J].Northern Horticulture,2012(10):42-44. |
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| Authors: | CHA Hui-ling SHI Yu-ping REN Shi-xia |
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| Institution: | 1.Qinghai Health Vocational and Technical College,Xining,Qinghai 810000;2.Department of Chemistry,Qinghai Normal University,Xining,Qinghai 810008;3.Qinghai Oilfield Drilling Technology Research Institute,Dunhuang,Gansu 736200) |
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| Abstract: | With Qinghai soft pear as test materials,used ethanol as extracting agent,extracting flavonoids by single and orthogonal test were studied.The results showed that optimal conditions for extraction was 95% ethanol concentration,extraction temperature was high at 80℃,the amount of ethanol was up to 90 mL and the extraction time was 3.0 h.Using Rutin as the standard,the total content of flavonoids was 0.0189 g/100g in soft pear pulp by spectrophotometry. |
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| Keywords: | soft pear flavonoids single test orthogonal test extraction |
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