| 辣椒三系子叶愈伤组织诱导及植株再生 |
| |
| 引用本文： | 宋利娜,康德贤,王红梅,王益奎,李文嘉,曹振木.辣椒三系子叶愈伤组织诱导及植株再生[J].北方园艺,2012(9):109-112. |
| |
| 作者姓名： | 宋利娜 康德贤 王红梅 王益奎 李文嘉 曹振木 |
| |
| 作者单位： | 广西大学农学院,广西南宁530004;广西农业科学院蔬菜研究所,广西南宁530007;广西作物遗传改良重点实验室,广西南宁530007;广西作物遗传改良重点实验室,广西南宁,530007;广西大学农学院,广西南宁,530004;广西农业科学院蔬菜研究所,广西南宁530007;广西作物遗传改良重点实验室,广西南宁530007;中国热带农业科学院热带作物品种资源研究所,海南儋州,571737 |
| |
| 基金项目： | 中央级公益性科研院所基本科研业务专项资金资助项目(PZS064);广西自然科学基金资助项目(2011GXNSFB018054) |
| |
| 摘 要： | 以辣椒雄性不育系、保持系、恢复系子叶为外植体,研究了6-BA、IAA浓度组合及AgNO3对子叶离体再生培养的影响。结果表明:三系之间在愈伤诱导和不定芽分化上均表现一定的差异,不仅最适的愈伤诱导和不定芽分化培养基不同,且分化出不定芽的数量也表现出较大差异,R-1分化出的不定芽数目明显多于A-1、B-1;AgNO3对愈伤组织诱导没有明显的作用,但3mg/L的AgNO3可提高不定芽的分化率;6-BA/IAA比值为10时与比值为4时相比更有利于不定芽的分化;1/2MS+IAA 0.2mg/L+NAA 0.1mg/L是辣椒三系最优的生根培养基配方。
|
| 关 键 词： | 植株再生 愈伤诱导 激素浓度 AgNO3 |
Plant Regeneration and Callus Induction of Three Lines of Peppers via Cotyledon Culture |
| |
| SONG Li-na
,
KANG De-xian
,
WANG Hong-mei
,
WANG Yi-kui
,
LI Wen-jia
,
CAO Zhen-mu.Plant Regeneration and Callus Induction of Three Lines of Peppers via Cotyledon Culture[J].Northern Horticulture,2012(9):109-112. |
| |
| Authors: | SONG Li-na KANG De-xian WANG Hong-mei WANG Yi-kui LI Wen-jia CAO Zhen-mu |
| |
| Institution: | 1.College of Agriculture,Guangxi University,Nanning,Guangxi 530004;2.Institute of Vegetable Research,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007;3.Key Laboratory of Guangxi Crop Genetic Improvement and Biotechnology,Nanning,Guangxi 530007;4.Institute of Tropical Crops Genetic Resources,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737) |
| |
| Abstract: | Taking male sterile line,maintainer line and restorer line of peppers cotyledon as explant,the factors effect of 6-BA,IAA concentration and AgNO3 on the in vitro regeneration culture of pepper cotyledon.The results showed that three lines of peppers had differences on callus and adventitious bud induction not merely the best medium of callus and adventitious bud induction but also the number of bud.The bud of R-1 easily outnumber A-1 and B-1;AgNO3 didn’t had an obvious effect on callus induction,but 3 mg/L AgNO3 could enhance the rate of adventitious bud differentiation;The higher 6-BA/IAA ratio of ten was conductive to the differentiation of the explants of cotyledons than it was four;1/2MS+IAA 0.2 mg/L+NAA 0.1 mg/L was the optimal medium of rooting by regenerated shoots. |
| |
| Keywords: | plant regeneration callus induction concentration of hormone AgNO3 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
