| 结球甘蓝西园4号种子纯度的SSR鉴定 |
| |
| 引用本文： | 苗明军,李成琼,司军,任雪松,宋洪元.结球甘蓝西园4号种子纯度的SSR鉴定[J].中国蔬菜,2011,1(2):53-55. |
| |
| 作者姓名： | 苗明军 李成琼 司军 任雪松 宋洪元 |
| |
| 作者单位： | 1.西南大学园艺园林学院，重庆市蔬菜学重点实验室，南方山地园艺学教育部重点实验室，重庆 400715；2四川省农业科学院园艺研究所，四川成都 610066 |
| |
| 摘 要： | 利用SSR分子标记技术对结球甘蓝西园4号及其亲本的DNA进行扩增，从90对SSR引物中筛选出1对具有双亲条带差异明显的引物O112-G04，构建了清晰的DNA扩增指纹图谱，并对西园4号种子进行了纯度鉴定，与田间形态学的鉴定结果高度一致，种子纯度均为96.7 %。结果表明，利用这对引物对结球甘蓝西园4号种子的纯度鉴定是可行的。
|
| 关 键 词： | 结球甘蓝 SSR标记 纯度鉴定 |
| 收稿时间： | 2010-06-25; |
Identification of Cabbage‘Xiyuan No.4’Seed Purity by SSR Markers |
| |
| MIAO Ming-jun,LI Cheng-qiong,SI Jun,REN Xue-song,SONG Hong-yuan.Identification of Cabbage‘Xiyuan No.4’Seed Purity by SSR Markers[J].China Vegetables,2011,1(2):53-55. |
| |
| Authors: | MIAO Ming-jun LI Cheng-qiong SI Jun REN Xue-song SONG Hong-yuan |
| |
| Institution: | 1.College of Horticulture and Landscape Architecture, Southwest University, Key Lab in Olericulture of Chongqing, Key Lab of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China;2.Horticulture Institute, Sichuan Academy of Agricultural Sciences Chengdu 610066, Sichuan, China |
| |
| Abstract: | Cabbage（Brassica oleracea L. var. capitata L.）‘Xiyuan No.4’and its parent were analyzed by SSR molecular marker technology. A primer, with obvious different bands parent, O112-G04 was screened from 90 pairs of SSR primers. The DNA fingerprinting of cabbage‘Xiyuan No.4’was constructed, and its seed purity was identified. The result is consistent with those obtained in the field tests. The seed purity was 96.7 %. This result shows that it is reliable to use this pair of SSR primer to identify cabbage‘Xiyuan No.4’seed purity. |
| |
| Keywords: | SSR marker Purity test |
|
| 点击此处可从《中国蔬菜》浏览原始摘要信息 |
| 点击此处可从《中国蔬菜》下载免费的PDF全文 |
|
