| KSOM无血清培养液在绵羊胚胎体外培养应用的研究 |
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| 引用本文： | 郑云胜,张家新,陈玉琦,李英,于凤玲.KSOM无血清培养液在绵羊胚胎体外培养应用的研究[J].内蒙古农业大学学报(自然科学版),2003,24(3):7-10. |
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| 作者姓名： | 郑云胜 张家新 陈玉琦 李英 于凤玲 |
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| 作者单位： | 1. 内蒙古农业大学动物与医学学院,呼和浩特,010018
2. 中国农业科学院畜牧研究所,北京,100094 |
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| 基金项目： | 国家自然科学基金(39460059) |
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| 摘 要： | 本文利用KSOM、TCM-199+20％人血清和KSOM+20％人血清培养绵羊体外受精胚胎,以研究KSOM作为绵羊胚胎体外培养无血清培养液的可能性。实验结果说明,KSOM支持绵羊胚胎早期发育至囊胚期,而在KSOM中添加血清后,胚胎可发育至孵化囊胚,而且孵化囊胚率高于常用的TCM-199+血清(24％vs 7％)(p<0.05)。说明KSOM可以用于绵羊胚胎早期发育的无血清培养液。
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| 关 键 词： | KSOM无血清培养液 绵羊 胚胎 体外培养 应用 体外受精胚胎 |
| 文章编号： | 1009-3575(2003)03-0007-04 |
STUDY ON USE OF KSOM AS SERUM FREE MEDIUM IN OVINE EMBRYO CULTURE IN VITRO |
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| ZHENG Yun-sheng,ZHANG Jia- xin,CHEN Yu- qi,LI Ying,YU Feng- lin.STUDY ON USE OF KSOM AS SERUM FREE MEDIUM IN OVINE EMBRYO CULTURE IN VITRO[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2003,24(3):7-10. |
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| Authors: | ZHENG Yun-sheng ZHANG Jia- xin CHEN Yu- qi LI Ying YU Feng- lin |
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| Institution: | ZHENG Yun-sheng~1,ZHANG Jia- xin~2,CHEN Yu- qi~1,LI Ying~1,YU Feng- lin~1 |
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| Abstract: | KSOM, TCM-199+20% human serum and KSOM+20% human serum was used as serum free medium in ovine embryo development. The results indicated that ovine embryos could develop to blastocyst in KSOM, and could develop to hatched blastocyst in TCM-199+20% human serum and KSOM+20% human serum. The rate of hatched blastocyst in KSOM+20% human serum was higher than that in TCM-199+20% human serum (24% vs 7% ) (P<0.05). This paper suggested that KSOM can be used as serum free medium for ovine embryo development in vitro. |
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| Keywords: | sheep embryo culture in vitro serum free medium KSOM |
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