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马铃薯试管苗对盐胁迫的生理反应
引用本文:崔焱森,张俊莲,李学才,王蒂,黄鹏,王丽,杜喜梅.马铃薯试管苗对盐胁迫的生理反应[J].中国马铃薯,2007,21(1):1-5.
作者姓名:崔焱森  张俊莲  李学才  王蒂  黄鹏  王丽  杜喜梅
作者单位:1. 甘肃农业大学农学院,甘肃,兰州,730070
2. 甘肃农业大学农学院,甘肃,兰州,730070;甘肃省作物遗传改良与种质创新重点实验室,甘肃,兰州,730070
3. 甘肃农业大学生命科学技术学院,甘肃,兰州,730070
基金项目:甘肃省科技攻关课题;甘肃省农业厅生物技术项目
摘    要:利用不同浓度的NaCl对马铃薯大西洋试管苗进行了20d胁迫。结果发现:随着盐浓度的增加,根和茎叶中Na+含量大幅度增加,K+含量基本稳定,Na+/K+呈极显著的上升趋势;叶片中叶绿素含量下降,但丙二醛含量增加,质膜透性增大,脯氨酸大量积累,且在一定盐度下脯氨酸含量与丙二醛含量和质膜透性间呈极显著的正相关,表明盐胁迫下脯氨酸积累的多少可反映马铃薯试管苗的受伤害程度。研究表明,Na+积累、膜透性增大和叶绿素含量下降是影响马铃薯试管苗生长的主要原因。

关 键 词:马铃薯  试管苗  盐胁迫  生理响应
文章编号:1672-3635(2007)01-0001-05
修稿时间:2006-09-24

Physiological Responses of Potato Plantlets in vitro to Salt Stress
Cui Yansen,Zhang Junlian,Li Xuecai,Wang Di,Huang Peng,Wang Li,Du Ximei.Physiological Responses of Potato Plantlets in vitro to Salt Stress[J].Chinese Potato,2007,21(1):1-5.
Authors:Cui Yansen  Zhang Junlian  Li Xuecai  Wang Di  Huang Peng  Wang Li  Du Ximei
Institution:1. Agronomy College, Gansu Agrieuhural University, Lanzhou, Gansu 730070, China; 2. Gansu Key Laboratory of Crop Genetic Improvement and Germplasm Enhancement, Lanzhou, Gansu 73b070, China; 3. Life Science and Technology College, Gansu Agricultural University, Lanzhou, Gansu 730070, China
Abstract:The potato cultivar Atlantic plantlets in vitro were treated with different concentrations of NaCl for 20 days.The results indicated that with more salt concentration the content of Na was increased dramatically,the content of K was stable and Na /K was increased significantly in the root and stem.In the leaf,the content of chlorophyll reduced,but the content of Malonaldehyde(MDA),membrane permeability and proline increased.Moreover,the content of proline was highly significantly correlated to the content of MDA and membrane permeability at a range of concentrations of NaCl,suggesting that the accumulation of proline could reflect the degree the plantlets in vitro got hurt.These reaults indicated that accumulated Na ,increase in membrane permeability and reduction in chlorophyll were the main effects on the growth of potato plantlets in vitro under salt stress.
Keywords:potato  plantlets in vitro  salt stress  physiological response
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