| 西瓜种子大小形状相关QTL分析 |
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| 引用本文： | 周慧文,卢丙洋,马鸿艳,高鹏,栾非时,高启帆,齐国安.西瓜种子大小形状相关QTL分析[J].园艺学报,2016,43(4):715-723. |
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| 作者姓名： | 周慧文 卢丙洋 马鸿艳 高鹏 栾非时 高启帆 齐国安 |
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| 作者单位： | （东北农业大学园艺学院，东北地区园艺作物生物学与种质创制农业部重点实验室，哈尔滨 150030） |
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| 基金项目： | 国家现代农业产业技术体系建设专项资金项目(CARS-26-02) |
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| 摘 要： | 以黏籽西瓜（Citrullus lanatus ssp. mucosospermus）‘PI186490’和普通栽培种西瓜（Citrullus lanatus ssp. vulgaris）‘LSW-177’为亲本，利用F2群体对种子大小和形状的QTL进行分析，构建了1张包含195个CAPS标记，并与染色体一一对应的11个连锁群的西瓜种子相关性状遗传连锁图谱。该图谱覆盖基因组长度2 029.8 cM，标记间平均距离10.46 cM。共检测到与种子相关性状QTL 23个，分布在连锁群LG1、LG2、LG3、LG5、LG6、LG8和LG11上，LOD值介于2.5208 ~ 36.0847之间，可解释2.2014% ~ 28.8950%的表型变异率。贡献率 > 17%的QTL位点4个，其中种子长度、种子宽度、种子厚度及种子百粒质量各1个。连锁群LG6定位到10个QTL，包含3个种子长度QTL、4个种子宽度QTL、1个种子厚度QTL及2个种子百粒质量QTL，4个贡献率 > 17%的QTL位点全部定位在连锁群LG6上，其中种子宽度和种子百粒质量主效QTL共同定位在标记WⅢ6-503和WⅢ6-502之间。
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| 关 键 词： | 西瓜 种子性状 CAPS 遗传连锁图谱 QTL |
QTL Mapping of Watermelon Seed Traits |
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| ZHOU Hui-wen,LU Bing-yang,MA Hong-yan,GAO Peng,LUAN Fei-shi,GAO Qi-fan,QI Guo-an.QTL Mapping of Watermelon Seed Traits[J].Acta Horticulturae Sinica,2016,43(4):715-723. |
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| Authors: | ZHOU Hui-wen LU Bing-yang MA Hong-yan GAO Peng LUAN Fei-shi GAO Qi-fan QI Guo-an |
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| Institution: | （Horticulture College，Northeast Agricultural University；Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（Northeast Region），Ministry of Agriculture，Harbin 150030，China） |
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| Abstract: | The inheritance of seed characteristic was studied by using the populations derived from the Egusi watermelon PI186490 and the cultivated watermelon LSW-177. A F2 genetic linkage map which included 195 CAPS markers was constructed. The map contained 11 linkage groups which corresponded with the chromosome and spanned 2 029.8 cM with a mean marker interval of 10.46 cM. Twenty-three QTLs for the seed traits were detected on LG1，LG2，LG3，LG5，LG6，LG8，LG11 and explained 2.2014%–28.8950% of the phenotypic variation. Of all the QTLs，4 QTLs for seed length，seed width，seed thickness and 100-seed weight explained over 17% of the phenotypic variation. Ten QTLs were identified on LG6，which contain 3 QTLs for seed length，4 QTLs for seed width，1 QTL for seed thickness and 2 QTLs for 100-seed weight. Four QTLs which explained over 17% of the phenotypic variation were also located on LG6 and QTL for seed width and 100-seed weight were both located on the two CAPS markers WⅢ6-503 and WⅢ6-502. |
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| Keywords: | watermelon seed trait CAPS genetic linkage map QTL |
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