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沙棘果废渣中原花青素提取纯化工艺研究
引用本文:田建华.沙棘果废渣中原花青素提取纯化工艺研究[J].山西林业科技,2011,40(3):11-13.
作者姓名:田建华
作者单位:山西省林业科学研究院,山西太原,030012
摘    要:以儿茶素为对照,沙棘原花青素得率为考察指标,通过正交试验法优选提取沙棘果废渣中原花青素的最佳工艺,并在此基础上,采用大孔树脂吸附、分离、纯化原花青素提取物。结果表明,超临界CO2脱脂后的沙棘果废渣中原花青素最佳提取工艺参数为:70%(体积分数)乙醇作溶剂,料液比1∶7,提取温度40℃,提取时间1 h;D-101型树脂对沙棘果废渣中原花青素吸附能力较强,纯化后的原花青素纯度可达73.3%.

关 键 词:沙棘  原花青素  提取  纯化

Study on Extraction and Purification of Procyanidins from Total Marc of Hippophae rhamnoides L.
Tian Jianhua.Study on Extraction and Purification of Procyanidins from Total Marc of Hippophae rhamnoides L.[J].Shanxi Forestry Science and Technology,2011,40(3):11-13.
Authors:Tian Jianhua
Institution:Tian Jianhua(Shanxi Academy of Forestry Sciences,030012 Taiyuan,China)
Abstract:The optimum process of extracting procyanidins from total marc of Hippophae rhamnoides L.was carried out by orthogonal test,and procyanidins was determined using catechin as reference substance.Extracts of total marc of sea buckthorn were isolated and purified by macroporous resin adsorption.The result shows that the optimum parameters of extraction is 70 %(volume fraction) ethanol,ratio of solid to liquid 1∶7,extraction temperature 40℃ and extraction time 1h.D-101 resin have more adsorption ability to procyanidins of total marc of Hippophae rhamnoides L.,the purity of procyanidins is 73.3%.
Keywords:Hippophae rhamnoides L    procyanidins  extraction  purification
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