| 粘膜免疫对鸡十二指肠SS mRNA表达的影响 |
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| 引用本文： | 杨倩,练高建.粘膜免疫对鸡十二指肠SS mRNA表达的影响[J].中国兽医学报,2003,23(3):284-286. |
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| 作者姓名： | 杨倩 练高建 |
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| 作者单位： | 南京农业大学,动物医学院,江苏,南京,210095 |
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| 基金项目： | 国家自然科学基金资助项目(30070564) |
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| 摘 要： | 应用鸡新城疫弱毒疫苗进行消化道粘膜免疫,或在肌肉注射生长抑素(SS)亚单位疫苗的基础上应用鸡新城疫弱毒疫苗进行消化道粘膜免疫,通过RT PCR方法检测免疫鸡十二指肠中SS基因的表达.结果表明,首免后第3周,新城疫免疫组的SS mRNA表达高于SS亚单位苗组,但各试验组无显著差异;首免后第4周,新城疫免疫组SS mRNA的表达显著高于SS亚单位苗组(P＜0.05)并高于对照组.提示粘膜免疫可促进小肠粘膜SS mRNA的表达,SS亚单位苗可在基因水平上降低SS mRNA的表达.
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| 关 键 词： | 粘膜免疫 十二指肠 SS mRNA 鸡 |
| 文章编号： | 1005-4545(2003)03-0284-02 |
| 修稿时间： | 2002年3月23日 |
Effect of mucosal immunization with newcastle attenuated vaccine on the expression of SS mRNA in chicken |
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| YANG Qian,LIAN Gao jian.Effect of mucosal immunization with newcastle attenuated vaccine on the expression of SS mRNA in chicken[J].Chinese Journal of Veterinary Science,2003,23(3):284-286. |
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| Authors: | YANG Qian LIAN Gao jian |
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| Abstract: | Broilers were immunized with Newcastle disease attenuated vaccine by digestive rout and immunized with NDV after intramuscular injection of somatostatin(SS) subunit vaccine to detect the expression of SS mRNA in the duodenum.The result suggested that the expression of SS mRNA in the duodenum was higher in NDV group than SS subunit vaccine group at 3 weeks after immunization,though no significant differences existed among all groups.The expression of SS mRNA in NDV group was higher than SS subunit vaccine and control group at 4 weeks after immunization in which the expression of SS mRNA in NDV group was significant higher than SS subunit vaccine group(P<0 05).Our result suggested that mucosal immunization can stimulate the expression of SS mRNA in the intestine of chicken and SS subunit vaccine may reduce the expression of SS mRNA. |
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| Keywords: | mucosal immunization expression SS mRNA chicken |
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