| 选择性培养基在小麦纹枯病菌分离及土壤菌核检测中的应用 |
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| 引用本文： | 杨岚,徐建强,王莉莉,任富豪,赵增新,李鹏飞,郑伟.选择性培养基在小麦纹枯病菌分离及土壤菌核检测中的应用[J].植物病理学报,2021,51(1):135-138. |
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| 作者姓名： | 杨岚 徐建强 王莉莉 任富豪 赵增新 李鹏飞 郑伟 |
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| 作者单位： | 河南科技大学林学院,洛阳 471003 |
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| 基金项目： | 河南省科技攻关(202102110071) |
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| 摘 要： | 由禾谷丝核菌(Rhizoctonia cerealis,Rc)引起的纹枯病是黄海麦区的重要病害1]。对病菌分离主要采用组织分离法2]。该法费时、费力、操作要求严格,且无法对土壤中的病菌数量进行检测。选择性培养基(Selective medium,SM)是根据不同真菌对化学药剂敏感性的不同,通过向基础培养基中添加特定的抑菌物质,达到专一性分离目标真菌而有选择地抑制杂菌生长的目的。
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| 关 键 词： | 小麦丝核菌 病原菌分离 病原菌检测 选择性培养基 |
| 收稿时间： | 2020-03-01 |
Application of selective medium to isolate Rhizoctonia cerealis and detect the number of sclerotinia in the wheat soil |
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| YANG Lan,XU Jian-qiang,WANG Li-li,REN Fu-hao,ZHAO Zeng-xin,LI Peng-fei,ZHENG Wei.Application of selective medium to isolate Rhizoctonia cerealis and detect the number of sclerotinia in the wheat soil[J].Acta Phytopathologica Sinica,2021,51(1):135-138. |
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| Authors: | YANG Lan XU Jian-qiang WANG Li-li REN Fu-hao ZHAO Zeng-xin LI Peng-fei ZHENG Wei |
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| Institution: | College of Forestry, Henan University of Science and Technology, Luoyang 471003, China |
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| Abstract: | The selective medium was based on WA medium, and added streptomycin sulfate, doxodine and carbendazim. The appropriate concentrations of three agents were 100 μg·mL-1, 1 μg·mL-1and 50 μg·mL-1, respectively. The selective culture medium for isolation of Rhizoctonia cerealis from the stem of infected wheat has the characteristics of time saving, labor saving and high success rate of isolation, and can be operated directly in non-sterile conditions; selective medium can be used to detect the sclerotia in soil, and provide some reference for predicting the occurrence and prevalence of wheat sharp eyespot. |
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| Keywords: | Rhizoctonia cerealis pathogen isolation pathogen detection selective medium |
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