| 水稻白化转绿和穗顶端退化突变体vpa1的遗传分析和基因定位 |
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| 引用本文： | 王中豪,贺彦,张晓波,徐霞,吴建利,施勇烽.水稻白化转绿和穗顶端退化突变体vpa1的遗传分析和基因定位[J].中国水稻科学,2021,35(1):19-26. |
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| 作者姓名： | 王中豪 贺彦 张晓波 徐霞 吴建利 施勇烽 |
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| 作者单位： | 中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室, 杭州310006; |
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| 基金项目： | 水稻生物学国家重点实验室自主课题资助项目(2017ZZKT10106)。 |
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| 摘 要： | 目的 通过对水稻转绿和穗顶端退化等突变体的研究,可以鉴定更多与叶绿体发育和穗发育相关的基因。方法 在常规种植条件下比较突变体vpa1（virescent and panicle abortion 1)表型及主要农艺性状差异,利用分离群体分析和图位克隆法进行相关基因定位。结果 突变体vpa1表现苗期白化,并逐渐转绿恢复成正常叶色,抽穗后可明显观查到穗顶端退化表型。vpa1的主要农艺性状除了结实率以外,株高、穗长、每穗实粒数等均较野生型显著下降。遗传分析表明白化转绿和穗顶端退化表型受独立的两个隐性基因控制。控制白化转绿叶性状的Osv16定位于第3染色体RM3441和RM3029之间约125kb物理区间内,区间内未见白化转绿性状相关基因的报道。控制穗顶端退化性状的Ospaa10定位于第1染色体RM11157和RM5972之间,区间内物理距离约190kb,区间内未见穗顶端退化相关基因的报道。结论 Osv16和Ospaa10两个基因的突变导致vpa1的叶色和穗型同时出现变异,为白化转绿基因Osv16和穗顶端退化基因Ospaa10的克隆和功能研究打下了基础。
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| 关 键 词： | 水稻 转绿 顶穗退化 基因定位 |
| 收稿时间： | 2020-07-02 |
| 修稿时间： | 2020-07-27 |
Genetic Analysis and Gene Mapping of a Virescent and Panicle Abortion Mutant vpa1 in Rice |
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| WANG Zhonghao,HE Yan,ZHANG Xiaobo,XU Xia,WU Jianli,SHI Yongfeng.Genetic Analysis and Gene Mapping of a Virescent and Panicle Abortion Mutant vpa1 in Rice[J].Chinese Journal of Rice Science,2021,35(1):19-26. |
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| Authors: | WANG Zhonghao HE Yan ZHANG Xiaobo XU Xia WU Jianli SHI Yongfeng |
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| Institution: | China National Center for Rice Improvement/ State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China |
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| Abstract: | 【Objective】Genes related to chloroplast development and panicle phenotype can be identified through the study on virescent mutants and panicle apical abortion (paa) mutants in rice. 【Method】The main agronomic traits of vpa1 (virescent and panicle abortion 1) mutant and the wild-type were compared under conventional planting conditions in field. The virescent gene and paa gene were mapped by bulked segregant analysis and map-based cloning.【Result】vpa1 exhibited albino leaves at seedling stage and the leaves gradually returned to normal. During heading stage, vpa1 showed degeneration of spikelets at the tops of panicles. Except seed-setting rate, the agronomic traits including plant height, panicle length, number of filled grains per panicle and 1000-grain weight decreased significantly in vpa1 as compared with the wild-type. Genetic analysis indicated that both the virescent phenotype and the paa phenotype of vpa1 are controlled by single recessive nuclear gene, and the virescent gene and paa gene were segregated independently. The virescent gene, Osv16, was mapped to a 125 kb region between RM3441 and RM3029 on chromosome 3. The paa gene, Ospaa10, was mapped to a 190 kb region between RM11157 and RM5972 on chromosome 1.【Conclusion】mutations of Osv16 and Ospaa10 caused simultaneous variation of leaf color and spikelet phenotype in vpa1. Osv16 and Ospaa10 are likely two new genes as no other virescent and paa genes have been identified in the related region, further cloning of both genes are currently underway. |
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| Keywords: | rice virescent panicle apical abortion gene mapping |
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