| 携带GFP的MNSV侵染性克隆载体的构建 |
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| 引用本文： | 吴会杰,彭斌,康保珊,刘丽锋,古勤生.携带GFP的MNSV侵染性克隆载体的构建[J].植物病理学报,2021,50(5):622-626. |
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| 作者姓名： | 吴会杰 彭斌 康保珊 刘丽锋 古勤生 |
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| 作者单位： | 中国农业科学院郑州果树研究所,郑州 450009 |
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| 基金项目： | 国家自然基金项目(31701941);河南省基础与前沿技术研究项目 (152300410231);中国农业科学院创新团队西甜瓜病虫害防控(CAAS-ASTIP-2018-ZFRI-08);国家西甜瓜产业体系病毒防控岗(CARS-26-13) |
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| 摘 要： |
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Construction of MNSV infectious clone harboring GFP gene |
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| WU Hui-jie,PENG Bin,KANG Bao-shan,LIU Li-feng,GU Qin-sheng.Construction of MNSV infectious clone harboring GFP gene[J].Acta Phytopathologica Sinica,2021,50(5):622-626. |
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| Authors: | WU Hui-jie PENG Bin KANG Bao-shan LIU Li-feng GU Qin-sheng |
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| Institution: | Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou450009, China |
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| Abstract: | Melon necrotic spot virus (MNSV) is a newly reported melon-infecting virus species in China, which is seriously terrible in melon production. The MNSV full-length cDNA of MNSV was obtained by RT-PCR and was ligated to pXT1 vector to obtain the infectious clone vector pXT1-MNSV. The melon was inoculated with pXT1-MNSV, and melon leaves infected with pXT1-MNSV were collected and inoculated into the healthy melon by sap. The results showed that the infectious clone pXT1-MNSV was well pathogenic and could be transmitted by sap, so the Koch′s law was completed. Using MNSV infectious clone, the MNSV infectious clone vector pMNSV-cp-GFP harboring full-length GFP was constructed, Because GFP fluorescence can be detected by RT-PCR and in both melon roots and leaves by confocal scanning laser microscopy. Here, We constructed MNSV infections clone and pMNSV-cp-GFP clone vectors for the first time in China. The MNSV infections clone provided a tool for the analysis of its pathogenic mechanism at the molecular level. In addition, we also constructed MNSV infectious clone vectors carrying GFP, which could be used in the fluorescent labeling of MNSV and its distribution, movement and colonization during virus infection. |
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| Keywords: | melon melon necrotic spot virus infectious clone recombination vector pathogenicity |
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