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引起河北省保定市白术根腐病的病原镰刀菌种类鉴定
引用本文:纪莉景,肖颖,李耀发,贾海民,王亚娇,吴玉星,孔令晓.引起河北省保定市白术根腐病的病原镰刀菌种类鉴定[J].植物病理学报,2021,51(2):282-286.
作者姓名:纪莉景  肖颖  李耀发  贾海民  王亚娇  吴玉星  孔令晓
作者单位:河北省农林科学院植物保护研究所,农业部华北北部作物有害生物综合治理重点实验室,河北省有害生物综合防治工程技术研究中心,保定 071000
基金项目:河北省财政项目(2019-1-1-3)
摘    要:

收稿时间:2020-04-02

Identification of pathogenic Fusarium species causing Atractylodes macrocephala root rot in Baoding,Hebei Province
JI Li-jing,XIAO Ying,LI Yao-fa,JIA Hai-min,WANG Ya-jiao,WU Yu-xing,KONG Ling-xiao.Identification of pathogenic Fusarium species causing Atractylodes macrocephala root rot in Baoding,Hebei Province[J].Acta Phytopathologica Sinica,2021,51(2):282-286.
Authors:JI Li-jing  XIAO Ying  LI Yao-fa  JIA Hai-min  WANG Ya-jiao  WU Yu-xing  KONG Ling-xiao
Institution:Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, China
Abstract:In order to identify the pathogenic fungi of Atractylodes macrocephala root rot in Baoding, Hebei Province, eighty strains of Fusarium species were isolated from 120 A. macrocephala plants with root rot symptoms with the isolation frequency at 66.7%. Fusarium species were identified by molecular techniques with specific primers, TEF-1α gene sequencing together with morphological characteristics. Thirty-nine F. solani strains were identified with the highest percentage at 48.8%, and 32 F. oxysporum strains were isolated with the percentage at 40.0%. Seven F. equiseti and two F. brachygibbosum strains accounted for 8.8% and 2.5%, respectively. The pathogenicity test on A. macrocephala showed that F. solani and F. oxysporum caused the typical symptoms of root rot. Koch's postulates were fulfilled following re-isolation and identification of the isolates of F. solani and F. oxysporum. Taken together, the results showed that F. solani and F. oxysporum were the pathogens of A. macrocephala root rot in Baoding, Hebei Province.
Keywords:Atractylodes macrocephala root rot  Fusarium oxysporum  Fusarium solani  pathogenicity  
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