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| 瓜类褪绿黄化病毒自然侵染南瓜及分子进化分析 | |
| 引用本文： | 刘放,刘勇,张德咏,刘嘉裕,李迅,戴良英,唐前君.瓜类褪绿黄化病毒自然侵染南瓜及分子进化分析[J].植物病理学报,2021,51(1):1-10. |
| 作者姓名： | 刘放 刘勇 张德咏 刘嘉裕 李迅 戴良英 唐前君 |
| 作者单位： | 湖南农业大学植物保护学院, 长沙 410128; 植物病虫害生物学与防控湖南省重点实验室, 长沙 410128; 湖南省农业科学院植物保护研究所, 长沙 410125; 美国爱荷华州立大学植物病理与微生物系 艾奥瓦州 50010 |
| 基金项目： | 国家公益性(农业)行业科技项目(201303028);国家重点研发计划项目(2017YFD0200908);湖南省教育厅开放基金项目(16K039);湖南省烟草公司科技计划项目(17-20ZDAa02) |
| 摘 要： | 瓜类褪绿黄化病毒(cucurbit chlorotic yellows virus,CCYV)可侵染多种瓜类,主要危害叶片,影响其光合作用,导致减产。采集湖南省南瓜疑似病毒病样品经siRNA测序发现CCYV,进一步RT-PCR验证CCYV可自然侵染南瓜,检出率为5.78%,这是我国内地首次发现CCYV可自然侵染南瓜。持续调查发现CCYV侵染湖南南瓜呈逐年上升趋势。克隆CCYV CP基因,进行系统发生、遗传变异、选择压力和基因漂流分析,发现CCYV种群分布呈明显地理相关性。自然选择和遗传漂变是CCYV进化的主要驱动力,中国种群存在正选择作用,CCYV正在发生适应性改变。CCYV种群数量正处于扩张状态。研究结果可为CCYV侵染南瓜的预警和防控提供科技支撑。 |
| 关 键 词： | 南瓜 瓜类褪绿黄化病毒 外壳蛋白 分子进化 自然侵染 |
| 收稿时间： | 2020-01-27 |
Molecular and evolutionary analysis of cucurbit chlorotic yellows virus naturally infecting pumpkin |
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| LIU Fang,LIU Yong,ZHANG De-yong,LIU Jia-yu,LI Xun,DAI Liang-ying,TANG Qian-jun.Molecular and evolutionary analysis of cucurbit chlorotic yellows virus naturally infecting pumpkin[J].Acta Phytopathologica Sinica,2021,51(1):1-10. | |
| Authors: | LIU Fang LIU Yong ZHANG De-yong LIU Jia-yu LI Xun DAI Liang-ying TANG Qian-jun |
| Institution: | College of Plant Protection,Hunan Agricultural University, Changsha 410128,China; Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Changsha 410128,China; Hunan Academy of Agricultural Sciences, Changsha 410125,China; Department of Plant Pathology & Microbiology, Iowa State University, Ames, Iowa 50010, The United States of America |
| Abstract: | Cucurbit chlorotic yellows virus (CCYV) can infect a variety of melons, mainly affecting the leaves and photosynthesis which ultimately results in reduced fruit weight. CCYV was detected by siRNA sequencing of suspected viral disease samples from Hunan province. Further analysis by RT-PCR confirmed that CCYV can naturally infect pumpkins with a detection rate of 5.78%. This is the first time that CCYV is being reported to naturally infect pumpkins in mainland China and it is found that the occurrence of pumpkins-infecting CCYV in Hunan province is increasing each year. The CCYV CP gene was cloned and analyzed for phylogeny, genetic differences, selection pressure and genetic drift. It was found that geographical distribution of the CCYV population was significantly correlated. Natural selection and genetic drift are the main drivers of CCYV evolution, but there is a positive selection effect in Chinese populations. Since CCYV is undergoing adaptive changes.CCYV population is expanding.Keeping in view the current spread of this virus, it might emerge as serious pathogen and cause more crop losses in the future. Thus, the results of our research are of significant importance for the early detection and prevention of CCYV infecting pumpkins. |
| Keywords: | pumpkin cucurbit chlorotic yellows virus coat protein molecular evolution natural infection |
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