| 妊娠早期饲粮添加不同水平N-氨甲酰谷氨酸改善母羊产羔性能的研究 |
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| 引用本文： | 蔡元,田见晖,王栋,罗玉柱,翁士乔,李春阳.妊娠早期饲粮添加不同水平N-氨甲酰谷氨酸改善母羊产羔性能的研究[J].畜牧兽医学报,2021,52(1):126-134. |
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| 作者姓名： | 蔡元 田见晖 王栋 罗玉柱 翁士乔 李春阳 |
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| 作者单位： | 1. 西北民族大学生命科学与工程学院, 兰州 730030;2. 中国农业大学动物科学技术学院, 北京 100193;3. 中国农业科学院北京畜牧兽医研究所, 北京 100193;4. 甘肃农业大学动物科学技术学院, 兰州 730070;5. 宁波三生生物科技有限公司, 宁波 315100 |
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| 基金项目： | “十三五”国家重点研发计划课题(2017YFD0501904);中央高校基本科研业务费专项资金项目(31920190043);甘肃农业大学课题(GSAU-ZL-2015-031);中央高校基本科研业务费专项资金项目(31920190208-02);兰州市科技计划项目(2016-3-91)。 |
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| 摘 要： | 旨在研究妊娠早期饲喂不同水平N-氨甲酰谷氨酸（N-carbamylglutamate，NCG）对母羊产羔性能及相关血液指标的影响。选用2~3胎次湖羊160只，随机分为4组，每组40只母羊，自配种当天（记为妊娠第0天）开始，分别饲喂基础饲粮（对照组）、基础饲粮+0.05% NCG、基础饲粮+0.08% NCG、基础饲粮+0.11% NCG，40 d后均饲喂基础饲粮。测定母羊产羔性能和妊娠第0、20、40天母羊血浆中游离氨基酸、总一氧化氮合酶（TNOS）、诱导型一氧化氮合酶（iNOS）、一氧化氮（NO）、生长激素、雌二醇、孕酮、尿素和氨浓度。结果显示，0.08%和0.11% NCG组产羔性能显著提高，两组的窝产羔数和窝产活羔数较对照组显著增加（P<0.05），但活羔初生个体重显著降低（P<0.05）；各NCG组妊娠第40天NO浓度显著升高（P<0.05），0.11% NCG组妊娠第40天iNOS浓度和妊娠第20天孕酮浓度较对照组显著升高（P<0.05）；0.08%和0.11% NCG组妊娠第20天氨浓度显著低于对照组和0.05% NCG组（P<0.05），0.11% NCG组妊娠第40天氨浓度显著低于对照组（P<0.05）。综上，妊娠早期母羊饲粮添加NCG可提高母羊体循环iNOS、NO和孕酮浓度，改善胎盘营养与妊娠维持状态；降低血浆氨浓度，提高胎儿发育环境安全性，改善母羊产羔性能，提高母羊窝产羔数和窝产活羔数。
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| 关 键 词： | N-氨甲酰谷氨酸 经产母羊 产羔性能 血液指标 |
| 收稿时间： | 2020-03-11 |
Effects of Dietary N-carbamylglutamate Supplementation on Improving Lambing Performance of Ewes during Early Pregnancy |
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| CAI Yuan,TIAN Jianhui,WANG Dong,LUO Yuzhu,WENG Shiqiao,LI Chunyang.Effects of Dietary N-carbamylglutamate Supplementation on Improving Lambing Performance of Ewes during Early Pregnancy[J].Acta Veterinaria et Zootechnica Sinica,2021,52(1):126-134. |
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| Authors: | CAI Yuan TIAN Jianhui WANG Dong LUO Yuzhu WENG Shiqiao LI Chunyang |
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| Institution: | 1. Life Science and Engineering College of Northwest Minzu University, Lanzhou 730030, China;2. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;3. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;4. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;5. Ningbo Sansheng Biotechnology Co., Ltd., Ningbo 315100, China |
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| Abstract: | The aim of the present study is to investigate the effects of dietary N-carbamylglutamate (NCG) supplementation on lambing performance and blood indexes of ewes during early pregnancy. A total of 160 second- and third-parity Hu sheep were randomly assigned into 4 groups which fed with basal diet(control group), basal diet supplemented with 0.05%, 0.08% and 0.11%NCG, respectively. Sheep were fed with experimental diets for 40 days from the day of mating. After that, all sheep were fed with basal diet. The parameters of lambing performance were recorded immediately after farrowing. The plasma amino acids, total nitric oxide synthase (TNOS), inducible nitric oxide synthase (iNOS), nitric oxide (NO), growth hormone, estradiol, progesterone, urea and ammonia were detected on the day 0, 20 and 40 of pregnancy. The results showed that the dietary supplementation of 0.08% and 0.11%NCG significantly increased the number of total lambs and total live lambs per litter compared with the control group(P<0.05), but the birth weight of lamb born alive decreased significantly (P<0.05). On day 40 of pregnancy, the plasma concentration of NO in NCG-supplemented groups were significantly higher than that of control group(P<0.05). In addition,compared to control group, 0.11% NCG-supplemented group had higher plasma concentration of iNOS on day 40 of pregnancy (P<0.05), as well as higher plasma concentration of progesterone on day 20 of pregnancy (P<0.05). Moreover, both 0.08% and 0.11% NCG -supplemented groups had lower level of plasma ammonia than those in control group and 0.05% NCG-supplemented group on day 20 of pregnancy (P<0.05), and on day 40 of pregnancy the plasma ammonia in 0.11% NCG-supplemented group was significantly lower than that of control group (P<0.05). The results suggested that dietary NCG supplementation during the early stage of pregnancy increased plasma concentration of iNOS, NO and progesterone, and thus improved placental nutrition and pregnancy maintenance. The reduced plasma concentration of ammonia by NCG supplementation, may improve the lambing performance by optimizing the uterine environment and enhancing fetal developmental potential. |
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| Keywords: | N-carbamylglutamate multiparous ewes lambing performance blood indexes |
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