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金线莲组培快繁技术
引用本文:何云芳,杨霞,余有祥.金线莲组培快繁技术[J].浙江林学院学报,1999,16(2):170-174.
作者姓名:何云芳  杨霞  余有祥
作者单位:浙江省林业厅种苗站,浙江省杭州市农业科学研究所
摘    要:用金线莲的茎段作外植体,比较不同的培养基,细胞分裂素,生长素,植物添加剂等因素对丛生芽生成的影响。实验证明B5及MS较好,BA的作用优于KT和ZT,最适质量浓度为4.0mg.L^-1,naa0.3mg.L^-1和GA30.3mg.L^-1对丛生芽的生成和伸长有较好的效果。

关 键 词:金线莲  组织培养  快速繁殖

Techniques of tissue culture and rapid propagation forAnoectochilus formosanus
HE Yunfang,YANG Xia,YU Youxiang,QIU Lizhen,JIANG Shengde.Techniques of tissue culture and rapid propagation forAnoectochilus formosanus[J].Journal of Zhejiang Forestry College,1999,16(2):170-174.
Authors:HE Yunfang  YANG Xia  YU Youxiang  QIU Lizhen  JIANG Shengde
Institution:HE Yunfang1,YANG Xia2,YU Youxiang1,QIU Lizhen1,JIANG Shengde1
Abstract:The cluster shoots and roots induced from stem segments of Anoectochilus formosanus intissue cultures was studied.The results showed that B5 and MS media,BA,NAA,GA,with the concentrations of 40 mgL-1,03 mgL-1,03 mgL-1 respectively,and LB were suitable for cluster buds generating and growing.1/10MS medium,20gL-1 sucrose and 05mgL-1 NAA were suitable for roots,and the segments vertically put on the surface of the medium rooted better.
Keywords:Anoectochilus formosanus  tissue    culturs  rapid propagation  
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