| 杀线虫细菌W3胞外体壁降解蛋白酶的纯化及部分特性 |
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| 引用本文： | 田宝玉,蓝灿华,江贤章,柯崇榕,黄薇,黄建忠.杀线虫细菌W3胞外体壁降解蛋白酶的纯化及部分特性[J].安徽农业科学,2009,37(24):11583-11586. |
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| 作者姓名： | 田宝玉 蓝灿华 江贤章 柯崇榕 黄薇 黄建忠 |
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| 作者单位： | 福建师范大学工业微生物教育部工程研究中心,福建,福州,350108 |
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| 基金项目： | 国家自然科学基金项目 |
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| 摘 要： | 目的]为筛选和开发高效新型生物杀线虫剂提供依据。方法]以土壤中筛选的1株具有明显杀线虫活性的细菌菌株W3为供试菌种，对其进行发酵培养，采用硫酸铵沉淀、疏水层析、阴离子柱层析等方法从发酵液中分离W3菌株的杀线虫活性成分，并对各活性成分的杀线虫活性进行测定。结果]W3菌株粗蛋白处理线虫4h后，虫体活动异常，部分虫体静止不动，处理36h后大部分虫体不活动，部分虫体体壁断裂现象，最终所有线虫被完全消解；从发酵液中纯化出1种能降解线虫体壁的胞外蛋白酶，该酶的分子量约为45KDa，其作用的最佳pH值为7～8，最佳温度为45℃。结论]W3菌株胞外蛋白酶具有明显的杀线虫活性和线虫体壁降解活性。
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| 关 键 词： | 杀线虫细菌W3 胞外蛋白酶 体壁降解 |
Purification of an Extracellular Cuticle Degrading Protease of Nematicidal Bacteria W3and its Partial Characteristics |
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| TIAN Bao-yu et al.Purification of an Extracellular Cuticle Degrading Protease of Nematicidal Bacteria W3and its Partial Characteristics[J].Journal of Anhui Agricultural Sciences,2009,37(24):11583-11586. |
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| Authors: | TIAN Bao-yu |
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| Institution: | TIAN Bao-yu et al(Engineering Research Center of Industrial Microbiology,Mministry of Education Fujian Normal University,Fuzhou,Fujian 350108) |
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| Abstract: | Objective] The aim was to provide the basis for screening and developing new type bio-nematicide Method] 1 bacterial strain W3 with obvious nematicidal activity was taken as the tested strain,it was conducted in fermentation culture,and its nematicidal activity compositions were separated with ammonium sulfate precipitation,hydrophobic interaction chromatography and anion column chromatography methods,the nematicidal activity of the active compositions were detected.Result] After 4 h of nematodes were tr... |
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| Keywords: | Nematicidal bacterium W3 Extracellular protease Cuticle degradation |
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