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双孢蘑菇褐变的酶学机理研究(续)
引用本文:池致念,柯家耀.双孢蘑菇褐变的酶学机理研究(续)[J].中国食用菌,1999,18(6):17-18.
作者姓名:池致念  柯家耀
作者单位:福建省轻工业研究所!福州350005(池致念,王泽生),福建省罐头行业协会!福州350001(柯家耀)
摘    要:从图谱可以看出,从蘑菇开始扭结成小米粒大到开伞整个过程中,其PPO同工酶图谱基本相似,但褐变程度却有些不同。在生长的开始阶段,电泳迁移率大的快泳区域(Rf0-68~0-70)具有较强的PPO酶活力,褐变程度亦深,但随着蘑菇生长,其活性强带主要是集中的Rf中等区域(Rf0-23~0-53),而随着蘑菇的成熟开伞,其活性强带主要是在Rf中慢泳区域(Rf0-23~0-37)。开伞菇薄菇始薄膜扣菇中菇小菇中豆菇小豆菇图2 蘑菇表层组织在常温中放置的PPO同工酶图谱2-5 蘑菇采后PPO酶活性变化 一般情…

关 键 词:双孢蘑菇  褐变  酶学机理

Study on the Enzymic Browning Mechanism of Agaricus bisporus
Chi Zhinian,Ke Jiayao,Wang Zesheng.Study on the Enzymic Browning Mechanism of Agaricus bisporus[J].Edible Fungi of China,1999,18(6):17-18.
Authors:Chi Zhinian  Ke Jiayao  Wang Zesheng
Abstract:The polyacrylamide slab gel electrophoresis(PAGE) was applied to make an exhaustive study on the browning mechanism of Agaricus bisporus.The result showed that,the polyphenol oxidase (PPO) was the main reason to cause mushroom brown after cropping.There were different enzymic activity and PAGE isozymic patterns from the defferent type of strains,the same as the different site of organism and different life stage during growing cycle.
Keywords:Agaricus bisporus  Browning  Enzyme  Mechanism  
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