| 过氧化氢酶与酵母菌共固定的研究 |
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| 引用本文： | 胡杨,雷女孝,王征,谢达平.过氧化氢酶与酵母菌共固定的研究[J].湖北农学院学报,2001,21(2):164-166. |
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| 作者姓名： | 胡杨 雷女孝 王征 谢达平 |
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| 作者单位： | 1. 湖南农业大学职教学院,
2. 湖南农业大学理学院, |
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| 基金项目： | 湖南省科技厅资助项目! (98- 2 5 - 3) |
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| 摘 要： | 采用聚阳离了复合物，用于过氧化氢酶与酵母细胞的共固定化，可防止使用过程中酶的渗漏，改善了供氧效果和酵母的增殖。固定化酶的最适反砂pH值范围为6－8。当聚阳离了浓度达150mmol/L时，固定化酶活力回收可达68．6％，在室温条件下，5d时酶活力下降58％。这种酶和酵母细胞的共固定颗粒，在1d时间内使颗粒内酵母细胞增殖至50亿／g。
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| 关 键 词： | 过氧化氢酶 酵母菌 共固定化 聚阳离子复合物 |
| 文章编号： | 1004-3888(2001)02-0164-03 |
| 修稿时间： | 2001年1月13日 |
Co-immobilizing of Catalase and Yeast |
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| HU Yang,LEI Xiao,WANG Zheng,XIE Da-ping.Co-immobilizing of Catalase and Yeast[J].Journal of Hubei Agricultural College,2001,21(2):164-166. |
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| Authors: | HU Yang LEI Xiao WANG Zheng XIE Da-ping |
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| Abstract: | Catalase and yeast were immobilized together by polycation complex.Catalase leakage was overcome in the doing so. Oxygen supply and yeast multiplication were improved. The optimum pH of the immobilizing was 6～8.The activity recovery of the immobilized enzyme reached 68% as polycation concentration was kept at 150 mmol/L.At room temperature,the activity of enzyme decreased about 58% in the 5th day.In the co-immobilizing particle yeast cell increased up to 5×109 /g in one day. |
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| Keywords: | catalase yeast co-immobilizing |
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