| 基于SRK和SCR序列的甘蓝自交不亲和系单倍型分析 |
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| 引用本文： | 高启国,韦静宜,朱利泉,王小佳.基于SRK和SCR序列的甘蓝自交不亲和系单倍型分析[J].西南农业大学学报,2011,33(2). |
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| 作者姓名： | 高启国 韦静宜 朱利泉 王小佳 |
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| 作者单位： | 教育部南方山地园艺学重点实验室西南大学重庆市蔬菜学重点实验室;西南大学植物生理生物化学实验室; |
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| 基金项目： | 国家自然科学基金项目(30900986); 重庆市自然科学基金项目(2009BB1298); 教育部博士点基金项目(200806350006); 西南大学博士基金项目(SWUB2008042)资助 |
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| 摘 要： | 采用RT-PCR和其他分子生物学方法,以甘蓝自交不亲和系E1和F1柱头cDNA为模板扩增SRKE1和SRKF1基因,以花药cDNA为模板扩增SCRE1和SCRF1基因.经序列比对首次证明E1和F1材料分别为S28和S7单倍型.经三维结构分析,预测SCRE1上C5-C6和C7-C8之间氨基酸序列决定SCRE1的单倍型特异性,SRKE1上TNNSFYSRLKVS序列决定了SRKE1的单倍型特异性,并对两者相对作用的关键氨基酸位点进行了分析.
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| 关 键 词： | 甘蓝 自交不亲和性 SRK基因 SCR基因 单倍型 |
Haplotype Analysis of Brassica oleracea Self-Incompatible Lines Based on SRK and SCR Sequences |
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| GAO Qi-guo,WEI Jing-yi,ZHU Li-quan,WANG Xiao-jia.Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education/Key Laboratory in Olericulture of Chongqing,Southwest University,Chongqing ,China,.Plant Physiology , Biochemistry Laboratory of Southwest University.Haplotype Analysis of Brassica oleracea Self-Incompatible Lines Based on SRK and SCR Sequences[J].Journal of Southwest Agricultural University,2011,33(2). |
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| Authors: | GAO Qi-guo WEI Jing-yi ZHU Li-quan WANG Xiao-jiaKey Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education/Key Laboratory in Olericulture of Chongqing Southwest University Chongqing China Plant Physiology Biochemistry Laboratory of Southwest University |
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| Institution: | GAO Qi-guo1,WEI Jing-yi1,ZHU Li-quan2,WANG Xiao-jia11.Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education/Key Laboratory in Olericulture of Chongqing,Southwest University,Chongqing 400716,China,2.Plant Physiology and Biochemistry Laboratory of Southwest University |
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| Abstract: | The cDNA fragment of SRK was amplified from the stigma cDNA of the self-incompatible lines of Brassica oleracea E1 and F1,and that of SCR was amplified from theor anther cDNA by RT-PCR and other methods of molecular biology.Based on sequence alignment,E1 and F1 were firstly proved to be S28 and S7 haplotype,respectively.Analysis of the three-dimensional structures of SCRE1 and SRKE1 suggested that the amino acid sequence between C5-C6 and C7-C8 of SCRE1 decides its haplotype specificity and the peptide of T... |
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| Keywords: | Brassica oleracea self-incompatibility S-locus receptor kinase(SRK) gene S-locus cysteine-rich protein(SCR) gene haplotype |
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