| 万古霉素分离纯化工艺研究 |
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| 引用本文： | 赵德,魏丽霞.万古霉素分离纯化工艺研究[J].西南农业大学学报,2011,33(3). |
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| 作者姓名： | 赵德 魏丽霞 |
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| 作者单位： | 北大国际医院集团重庆大新药业股份有限公司;西南大学药学院; |
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| 摘 要： | 研究了从发酵液中纯化万古霉素的工艺.联合应用阳离子交换树脂法和大孔树脂吸附色谱法,通过比较各备选树脂的载样量、洗脱回收率、纯化倍数,优选树脂型号,确定了万古霉素纯化工艺:发酵液上PK206树脂柱,用0.1 mol/L的NH4OH溶液洗脱,收集第4,5部分(0.5 BV/部分)上CM650树脂柱,用0.3 mol/L的NH4Cl溶液洗脱,收集第6-10部分(0.5 BV/部分).收集液经纳滤脱盐、浓缩,最后产品的纯度达93.44%,总回收率为64.73%.
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| 关 键 词： | 万古霉素 纯化 阳离子交换树脂 大孔吸附树脂 |
Studies on the Separation and Purification Technology of Vancomycin |
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| ZHAO De,WEI Li-xia .PKU International Health Care Group Chongqing Daxin Pharmaceutical CO.,Ltd.,Chongqing ,China,.School of Pharmaceutical Sciences,Southwest University,Chongqing.Studies on the Separation and Purification Technology of Vancomycin[J].Journal of Southwest Agricultural University,2011,33(3). |
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| Authors: | ZHAO De WEI Li-xia PKU International Health Care Group Chongqing Daxin Pharmaceutical CO Ltd Chongqing China School of Pharmaceutical Sciences Southwest University Chongqing |
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| Institution: | ZHAO De1,WEI Li-xia2 1.PKU International Health Care Group Chongqing Daxin Pharmaceutical CO.,Ltd.,Chongqing 400700,China,2.School of Pharmaceutical Sciences,Southwest University,Chongqing 400716 |
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| Abstract: | The separation and purification technology of vancomycin from the fermentation liquor was studied with cation exchange resins in combination with macroporous adsorptive resins.Several types of cation exchange resins and macroporous adsorptive resins were compared on the parameters of load carrying capacity,recovery rate and purification fold to select the optimum resins.The purification technology for vancomycin was identified as follows: the fermentation liquor of vancomycin was exchanged onto PK206 resin ... |
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| Keywords: | vancomycin purification cation exchange resin macroporous adsorptive resin |
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