| 植物生物反应器中重组蛋白产量低的原因及对策 |
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| 引用本文： | 丁勇,龚秀会,刘小烛.植物生物反应器中重组蛋白产量低的原因及对策[J].安徽农学通报,2009,15(21):41-42. |
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| 作者姓名： | 丁勇 龚秀会 刘小烛 |
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| 作者单位： | 西南林学院资源学院,云南昆明,650224;西南林学院资源学院,云南昆明,650224;西南林学院资源学院,云南昆明,650224 |
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| 基金项目： | 西南地区生物多样性保育国家林业局重点实验室开放基金项目，云南省重点建设专业西南林学院林学专业项目联合资助 |
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| 摘 要： | 包括种子蛋白质贮藏液泡、种子油体、植物悬浮培养细胞、毛状根和叶绿体在内的植物生物反应器作为外源蛋白表达体系尽管已经获得了成功,但迄今为止,在植物生物反应器中仍存在重组蛋白产量低的关键问题。原因可能是重组蛋白在植物表达体系中容易被降解。因此,一种可能的解决方法就是,在转基因植物中将目标蛋白与蛋白酶抑制剂进行共表达,保护其不被降解,从而获得高产量的重组蛋白。
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| 关 键 词： | 植物生物反应器 重组蛋白 蛋白酶抑制剂 共表达 |
Cause and Improvement of the Low Yield of Recombinant Proteins in Plant Bioreactors |
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| Ding Yong et al..Cause and Improvement of the Low Yield of Recombinant Proteins in Plant Bioreactors[J].Auhui Agricultural Science Bulletin,2009,15(21):41-42. |
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| Authors: | Ding Yong |
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| Institution: | Ding Yong et al. (College of Natural Resources,Southwest Forestry University,Kunming 650224,China) |
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| Abstract: | Even though various successful expression systems have been reported in plant bioreactors including the use of seed protein storage vacuoles or oil bodies,cell suspension cultures,root exudates and chloroplasts,a critical problem in plant bioreactors thus far is the low yield of the recombinant proteins due to the degradation system in plants.As a result,one of the possible solution or improvement is to co-express the target protein together with protease inhibitor to protect the protein from being degradat... |
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| Keywords: | Plant bioreactors recombinant proteins protease inhibitor co-expression |
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