| 芝麻EST-SSR引物的开发与应用 |
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| 引用本文： | 柯涛,毛晗,董彩华,陈书元,于景印,赵应忠,刘红艳,刘胜毅.芝麻EST-SSR引物的开发与应用[J].中国油料作物学报,2013,35(1):43-47. |
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| 作者姓名： | 柯涛 毛晗 董彩华 陈书元 于景印 赵应忠 刘红艳 刘胜毅 |
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| 作者单位： | 农业部油料作物生物学与遗传育种重点实验室 中国农业科学院油料作物研究所;南阳师范学院生命科学与技术学院 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)(2011CB109300);湖北农业科技创新中心;农业部油料作物生物学重点开放实验室开放课题(201202);中国农业科学院油料作物研究所所长基金(1610172011011) |
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| 摘 要： | 为丰富芝麻EST-SSR引物，本研究从实验室构建的高油芝麻品系中芝14号的不同种子发育期cDNA文库中获得45 569条表达序列标签（expressed sequence tags，EST），通过前处理得到总长17.428Mb的无冗余EST共32 421条。利用MISA和SSR finder软件包工具对EST序列进行SSR位点查找，在这些序列中发现有1 688条Unigene，共有1 949个SSR。共226个序列含2个或2个以上SSR位点。这些SSR中出现频率最高的重复基序类型为三核苷酸(74%)。为开发芝麻EST-SSR引物，探讨EST-SSR用于芝麻品种遗传差异研究的可行性，用12份不同含油量品系材料评价其中34对与油脂代谢相关的基因SSR引物，全部成功扩增，共有3对引物获得多态性条带。结果表明，芝麻EST-SSR 标记开发效率较高，是芝麻SSR标记开发的重要措施，对于芝麻品种的鉴定与遗传多样性分析具有很高的应用价值。
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| 关 键 词： | 芝麻 EST-SSR 油脂代谢 SSR标记 |
Development and application of EST-derived SSR markers for sesame |
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| KE Tao,MAO Han,DONG Cai-hua,CHEN Shu-yuan,YU Jing-yin,ZHAO Ying-zhong,LIU Hong-yan,LIU Sheng-yi.Development and application of EST-derived SSR markers for sesame[J].Chinese Journal of Oil Crop Sciences,2013,35(1):43-47. |
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| Authors: | KE Tao MAO Han DONG Cai-hua CHEN Shu-yuan YU Jing-yin ZHAO Ying-zhong LIU Hong-yan LIU Sheng-yi |
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| Institution: | 1*(1.Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture, Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China; 2.Department of Life Science and Technology,Nanyang Normal University,Nanyang 473061,China) |
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| Abstract: | Currently, the lack of EST sequence information has limited the discovery of sesame EST-SSR primer number. All the 45569 EST sequences of high oil content sesame species Zhongzhi14 were obtained from a full-length developments seeds cDNA library and assembled 32421 unigenes. The unigenes represented about 17.428 Mb of genic sequences. Using MISA and SSR finder software to screening SSRs, 1949 non-redundant (NR) SSRs were identified in 1688 uniESTs. A total of 226 sequences contained more than one SSR. The trinucleotide repeats are the dominant types with the frequency of 74%. All of the designed 34 SSR primer pairs related to oil synthesis genes could amplify characteristic SSR fragments against 12 different oil content species, and 3 primer pairs amplified polymorphic fragments. The EST-SSR markers of sesame are highly efficiency, and should be the ideal tools for studies of genetic variation, genetic diversity, and development of genetic linkage maps of sesame. |
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