| 水稻根际微生物肠杆菌E-25和肺炎克雷伯氏菌K-12固氮作用的一些生理特性 |
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| 引用本文： | 徐继,王继文,辛淑英,王发珠,李佳格.水稻根际微生物肠杆菌E-25和肺炎克雷伯氏菌K-12固氮作用的一些生理特性[J].华北农学报,1987(3). |
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| 作者姓名： | 徐继 王继文 辛淑英 王发珠 李佳格 |
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| 作者单位： | 中国科学院植物研究所 北京
(徐继，王继文，辛淑英，王发珠)，中国科学院植物研究所 北京(李佳格) |
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| 摘 要： | 我们研究了从京郊地区水稻根际分离出的一株肠杆菌(Enterob acter sp)E-25和一株肺炎克雷伯氏菌(Klebsiella pneumoniae)K-12的固氮特性.两种菌均具有较强的固氮活力.用乙炔还原法测定,E-25的比活为552毫微克分子C_2H_4/小时.毫克菌体蛋白,K-12的比活为676毫微克分子C_2H_4/小时.毫克菌体蛋白.在对数生长期菌体蛋白迅速增长,固氮活性也随之增高.对数生长期后,菌体蛋白停止增长,固氮活性开始下降.E-25和K-12均为兼性好氧细菌,在无氧和微氧条件下固氮活力显著高于常氧条件下.E-25比K-12对氧更加敏感.这两种菌可能是在北方碱性土壤中占据优势的根际固氮细菌.由于长期适应生存条件的结果,这两种菌固氮的最适pH值均偏碱性.E-25对碳源的利用也不同于南方水稻根际分离出的肠杆菌.E-25只能利用果糖、葡萄糖和蔗糖,而不能利用琥珀酸、柠檬酸等有机酸做固氮的能源.我们推测E-25在固氮过程中所需能量主要来自糖酵解途经.
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| 关 键 词： | 水稻根际微生物 固氮作用 |
SOME PROPERTIES OF NITROGEN FIXATION BY EMTEROBACTER SP. 25(E-25) AND KLEBSIELLA PNEUMONIAE 12(K-12) ISOLATED FROM RHIZOSPHERE OF RICE |
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| Xu Ji,Wang Jiwen,Xin Shuying,Wang Fazhu,Li Jiage.SOME PROPERTIES OF NITROGEN FIXATION BY EMTEROBACTER SP. 25(E-25) AND KLEBSIELLA PNEUMONIAE 12(K-12) ISOLATED FROM RHIZOSPHERE OF RICE[J].Acta Agriculturae Boreali-Sinica,1987(3). |
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| Authors: | Xu Ji Wang Jiwen Xin Shuying Wang Fazhu Li Jiage |
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| Abstract: | Two strains of bacteria E-25 and K-12 were isolated from rice rootsgrowing in Beijing area. Both E-25 and K-12 possessed significant capability of nitrogen fixation. The maximum nitrogenase activity measured by acetylene reduction assay of E-25 was 552 nmol C2H4/hr/mg protein, and K-12 was676 nmol C2H4/hr/mg protein. During the exponential growth, the total cell protein as well as nitrogenase activity increased rapidly. After the exponential growth, nitrogenase activity declined as the increase of cell protein stopped. The nitrogenase activity of E-25 and K-12, which are faculative aerobic bacteria, were higher under anaerobic or low oxygen level than under high oxygen level, while E-25 was more sensitive to oxygen than K-12. The E-25 associated with N2-fixing bacteria, isolated from rice root growing in alkaline soil in Beijing area, significantly differed from that in southern China acidic soil in the utilization of carbon source. The former uses fructose, glucose and sucrose as energy sources for nitrogen fixation, but does not use succinate and citrate. It seems that habitat difference causes the diversity of these bacteria. It issuggested that the energy for nitrogen fixation of E-25 may come from the glycalytic pathway. |
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| Keywords: | Rice Rhzosphere microorganism Nitrogen fixation |
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