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Mass production of competent larvae of the sea urchin <Emphasis Type="Italic">Lytechinus variegatus</Emphasis> (Echinodermata: Echinoidea)
Authors:Email author" target="_blank">Esperanza?BuitragoEmail author  Cèsar?Lodeiros  Karem?Lunar  Daniela?Alvarado  Federico?Indorf  Khenia?Frontado  Pulido?Moreno  Zoila?Vasquez
Institution:(1) Fundación La Salle de Ciencias Naturales (FLASA), Estación de Investigaciones Marinas de Margarita (EDIMAR), Porlamar, 6301, Apartaao Postal 144, Isla Margarita, Estado Nueva Esparta, Venezuela;(2) Instituto Oceanográfico de Venezuela, Universidad de Oriente, 6101 Cumaná, Estado Sucre, Venezuela
Abstract:We evaluated the mass production of competent larvae of the sea urchin Lytechinus variegatus cultured at three initial densities (0.25, 0.5, and 1 larvae per ml) and fed Chaetoceros muelleri. Survival, length, dry weight of larvae, and larval stage index (LSI) were estimated in each treatment as a function of the density. Density decreased during the experiment due to mortality, but the percentage was similar in all three treatments (68.5, 66.7, and 76.0%). The experiment was stopped at 13 days after fertilization, when most of the larvae were competent and had settled. There were no significant differences in survival (exceeded 65% in all treatments), length and larval stage index among treatments. However, larvae weight in the two low density treatments (1.1 ± 0.11 mg and 1.2 ± 0.05 mg, respectively) was greater than the high density treatment (0.59±0.376 mg). This study demonstrates that competent larvae of Lytechinus variegatus can be produced with less than 25% mortality in 13 days when cultures are started at densities of 0.25–1 larvae/ml. Culturing at higher densities (0.5–1 larvae/ml) had no apparent disadvantages and would reduce the cost of production.
Keywords:Culture  Density  Echinoid  Larvae  Lytechinus variegatus
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