| 鹅掌楸种间杂交组合叶芽发育时期基因表达的差异 |
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| 引用本文： | 李帅,徐进,李火根,施季森.鹅掌楸种间杂交组合叶芽发育时期基因表达的差异[J].东北林业大学学报,2011,39(5):1-3. |
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| 作者姓名： | 李帅 徐进 李火根 施季森 |
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| 作者单位： | 林木遗传与生物技术省部共建教育部重点实验室(南京林业大学); |
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| 基金项目： | 江苏省自然科学基金项目(BK2008051); 国家自然科学基金项目(NSFC30930077) |
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| 摘 要： | 以鹅掌楸属植物正反交2个组合为材料,利用DDRT-PcR技术,研究其在不同生长发育阶段基因表达的差异.结果表明:2组合总条带表达数都是在生长旺盛期最大,休眠期次之,萌动期最小.杂种鹅掌楸H×M和BM×J的双亲共沉默型(Ⅰ)、杂种特异表达型(Ⅱ)在不同生长时期均存在极显著差异.而单亲表达一致型(Ⅲ)、单亲表达沉默型(Ⅳ)...
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| 关 键 词： | 鹅掌楸属 生长发育期 基因差异表达 DDRT-PCR技术 |
Gene Differential Expression in Leaf Bud of Interspecific Hybrids in Liriodendron During Different Growth and Development Stages |
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| Li Shuai,Xu Jin,Li Huogen,Shi Jisen.Gene Differential Expression in Leaf Bud of Interspecific Hybrids in Liriodendron During Different Growth and Development Stages[J].Journal of Northeast Forestry University,2011,39(5):1-3. |
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| Authors: | Li Shuai Xu Jin Li Huogen Shi Jisen |
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| Institution: | Li Shuai,Xu Jin,Li Huogen,Shi Jisen(Key Laboratory of Forest Genetics and Biotechnology(Nanjing Forestry University),Ministry of Education,Nanjing 210037,P.R.China) |
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| Abstract: | An experiment was conducted to study gene expression differences of the reciprocal crosses of Liriodendron during different growth and development stages by DDRT-PCR.Results showed that the total band quantities of the both crosses were the largest in the vigorous stage,followed by the dormant stage and budding stage in order.There were significant differences in type-Ⅰ(bands present in only parents) and type-Ⅱ(bands present in only both hybrids) of Liriodendron hybrids H×M and BM×J among different growth and development stages,but there were no significant differences in type-Ⅲ(bands present in both hybrids and one parent) and type-Ⅳ(bands present in only one parent) of Liriodendron hybrids H×M and BM×J.Significant differences in differential gene expression patterns(Ⅰ,Ⅱ,Ⅲ,Ⅳ) of Liriodendron hybrids H×M and BM×J were observed among budding stage,vigorous stage and dormant stage. |
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| Keywords: | Liriodendron Growth and development stages Differential gene expression |
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